Evidence map›Paper›PMID 2673120›Full record

ArticleArchives of microbiology1989

Cloning of the LEU2 gene of Saccharomyces cerevisiae by in vivo recombination.

R Valinger, G Braus, P Niederberger, M Künzler, G Paravicini, T Schmidheini, R Hütter

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

R ValingerMikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
G Braus
P Niederberger
M Künzler
G Paravicini
T Schmidheini
R Hütter
Board of the Swiss Federal Institutes of Technology · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombination results preferentially in a plasmid that carries both the LEU2-d and the entire LEU2 gene. In addition we also found one plasmid with a tandem LEU2 insertion and one plasmid where the LEU2-d gene was replaced by the entire LEU2 gene.

Indexed as

Cloning, MolecularGenes, FungalRecombination, GeneticBase SequenceBlotting, SouthernEscherichia coliLeucineMolecular Sequence DataNucleic Acid HybridizationPlasmidsRestriction MappingSaccharomyces cerevisiaeLeucine

Identifiers

PMID2673120
OpenAlexW2070202126

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.