Trial reportPloS one2016
Impact of Denture Cleaning Method and Overnight Storage Condition on Denture Biofilm Mass and Composition: A Cross-Over Randomized Clinical Trial.
Trial report in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02454413 (ASSESSMENT OF THE IMPACT OF CLEANING METHODS AND OVERNIGHT DENTURE STORAGE ON BIOFILM FORMATION ON REMOVABLE ACRYLIC DENTURES.), which is not on this map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Assessment of the impact of cleaning methods and overnight denture storage on biofilm formation on removable acrylic dentures.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Denture Plaque Biofilm Visual Assessment Methods: A Systematic Review.International dental journal · 2024Pooled it
- Effectiveness of Cleaning Methods for Resilient Denture Liners: A Three-Period Randomized Crossover Trial.Clinical and experimental dental research · 2025Trial
- Anti-biofilm effectiveness of protocols for cleaning complete dentures in hospitalized patients: a randomized controlled trial.Journal of applied oral science : revista FOB · 2024Trial
- Denture-associated oral microbiome in dentate and edentulous older adults living in long-term care facilities.Journal of oral microbiology · 2026Article
- Antimicrobial Resistance in Oral Healthcare: A Growing Concern in Dentistry.Dentistry journal · 2025Review
- Article
- The effect of various denture cleansers on the physical and mechanical properties of CAD/CAM and heat-polymerized denture base materials: an in vitro study.Clinical oral investigations · 2024Article
- The effect of attachment systems and denture cleaning methods on microbial biomass and composition in implant-supported overdentures: an experimental study.International journal of implant dentistry · 2024Article
- Nanoparticle-Modified 3D-Printed Denture Base Resins: Influence of Denture Cleansers on the Color Stability and Surface Roughness In Vitro.Nanomaterials (Basel, Switzerland) · 2024Article
- Strategies for Preventing and Treating Oral Mucosal Infections Associated with Removable Dentures: A Scoping Review.Antibiotics (Basel, Switzerland) · 2024Article
- Review
- In vitro efficacy of synthetic lawsone derivative disinfectant solution on removing dual-species biofilms and effect on acrylic denture surface properties.Scientific reports · 2023Article
- A randomized trial of the effectiveness of an ultrasonic denture hygiene intervention program among community dwelling elders.European oral research · 2023Article
- Effects of Denture Cleaning Regimens on the Quantity ofInternational journal of environmental research and public health · 2022Article
- An In Vitro Evaluation of Denture Cleansing Regimens against a Polymicrobial Denture Biofilm Model.Antibiotics (Basel, Switzerland) · 2022Article
- The effect of hydrochloric acid and sodium hypochlorite on fracture resistance of orthodontic self-cure acrylic base.Dental research journal · 2022Article
- Removable Denture Wearing as a Risk Predictor for Pneumonia Incidence and Time to Event in Older Adults.JDR clinical and translational research · 2021Article
- Pneumonia prevention in the elderly patients: the other sides.Aging clinical and experimental research · 2021Article
- Comparison of Antifungal Efficacy of Zataria Multiflora and Nystatin for Treatment of Denture Stomatitis: A Randomized Clinical Trial.Journal of dentistry (Shiraz, Iran) · 2021Article
- Oral Hygiene Habits of Complete Denture Wearers in Central Transylvania, Romania.Oral health & preventive dentistry · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAppropriate oral hygiene is required to maintain oral health in denture wearers. This study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures.
methodsIn a cross-over randomized controlled trial in 13 older people, 4 conditions with 2 different mechanical cleaning methods and 2 overnight storage conditions were considered: (i) brushing and immersion in water without a cleansing tablet, (ii) brushing and immersion in water with a cleansing tablet, (iii) ultrasonic cleaning and immersion in water without a cleansing tablet, and (iv) ultrasonic cleaning and immersion in water with a cleansing tablet. Each test condition was performed for 5 consecutive days, preceded by a 2-days wash-out period. Biofilm samples were taken at baseline (control) and at the end of each test period from a standardized region. Total and individual levels of selected oral bacteria (n = 20), and of Candida albicans were identified using the Polymerase Chain Reaction (PCR) technique. Denture biofilm coverage was scored using an analogue denture plaque score. Paired t-tests and Wilcoxon-signed rank tests were used to compare the test conditions. The level of significance was set at α< 5%.
resultsOvernight denture storage in water with a cleansing tablet significantly reduced the total bacterial count (p<0.01). The difference in total bacterial level between the two mechanical cleaning methods was not statistically significant. No significant effect was observed on the amount of Candida albicans nor on the analogue plaque scores.
conclusionsThe use of cleansing tablets during overnight denture storage in addition to mechanical denture cleaning did not affect Candida albicans count, but reduced the total bacterial count on acrylic removable dentures compared to overnight storage in water. This effect was more pronounced when combined with ultrasonic cleaning compared to brushing.
trial registrationClinicalTrials.gov NCT02454413.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.