ArticlePloS one2016
Experimental Genome-Wide Determination of RNA Polyadenylation in Chlamydomonas reinhardtii.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- A kingdom-wide full-length RNA atlas reveals the evolutionary landscapes of plant poly(A) tails.Genome biology · 2026Article
- Poly(A) tail regulation in stem cells and early development.Cell regeneration (London, England) · 2025Review
- Nucleotide-resolution Mapping of RNA N6-Methyladenosine (m6A) modifications and comprehensive analysis of global polyadenylation events in mRNA 3' end processing in malaria pathogenbioRxiv : the preprint server for biology · 2025Article
- Article
- Alternative Polyadenylation Is a Novel Strategy for the Regulation of Gene Expression in Response to Stresses in Plants.International journal of molecular sciences · 2023Review
- Phylogenetic analysis and structural prediction reveal the potential functional diversity between green algae SWEET transporters.Frontiers in plant science · 2022Article
- Exploring the Impact of Terminators on Transgene Expression inLife (Basel, Switzerland) · 2021Article
- Identification of Alternative Polyadenylation inFrontiers in genetics · 2021Article
- Article
- Profiling Alternative 3' Untranslated Regions in Sorghum using RNA-seq Data.Frontiers in genetics · 2020Article
- Pipecolic acid confers systemic immunity by regulating free radicals.Science advances · 2018Article
- Characterization of mRNA polyadenylation in the apicomplexa.PloS one · 2018Article
- Noncanonical Alternative Polyadenylation Contributes to Gene Regulation in Response to Hypoxia.The Plant cell · 2017Article
- PlantAPA: A Portal for Visualization and Analysis of Alternative Polyadenylation in Plants.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polyadenylation of RNA is a near-universal feature of RNA metabolism in eukaryotes. This process has been studied in the model alga Chlamydomonas reinhardtii using low-throughput (gene-by-gene) and high-throughput (transcriptome sequencing) approaches that recovered poly(A)-containing sequence tags which revealed interesting features of this critical process in Chlamydomonas. In this study, RNA polyadenylation has been studied using the so-called Poly(A) Tag Sequencing (PAT-Seq) approach. Specifically, PAT-Seq was used to study poly(A) site choice in cultures grown in four different media types-Tris-Phosphate (TP), Tris-Phosphate-Acetate (TAP), High-Salt (HS), and High-Salt-Acetate (HAS). The results indicate that: 1. As reported before, the motif UGUAA is the primary, and perhaps sole, cis-element that guides mRNA polyadenylation in the nucleus; 2. The scope of alternative polyadenylation events with the potential to change the coding sequences of mRNAs is limited; 3. Changes in poly(A) site choice in cultures grown in the different media types are very few in number and do not affect protein-coding potential; 4. Organellar polyadenylation is considerable and affects primarily ribosomal RNAs in the chloroplast and mitochondria; and 5. Organellar RNA polyadenylation is a dynamic process that is affected by the different media types used for cell growth.
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Registered trials
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