ArticleNeurophotonics2015
Ultramicroscopy: development and outlook.
Article in Neurophotonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- The 15th Anniversary ofMaterials (Basel, Switzerland) · 2025Article
- Article
- Review
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- EDTP enhances and protects the fluorescent signal of GFP in cleared and expanded tissues.Scientific reports · 2024Article
- Tutorial: practical considerations for tissue clearing and imaging.Nature protocols · 2021Review
- Hypoglossal Nerve Abnormalities as Biomarkers for Central Nervous System Defects in Mouse Lines Producing Embryonically Lethal Offspring.Frontiers in neuroanatomy · 2021Article
- Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy.Nature protocols · 2020Review
- Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy.Neuron · 2020Review
- Multiscale light-sheet for rapid imaging of cardiopulmonary system.JCI insight · 2018Review
- Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair.Current cardiology reports · 2018Review
- Integrating light-sheet imaging with virtual reality to recapitulate developmental cardiac mechanics.JCI insight · 2017Article
- Multimode fibre based imaging for optically cleared samples.Biomedical optics express · 2017Article
- Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue.Biomedical optics express · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We present an overview of the ultramicroscopy technique we developed. Starting from developments 100 years ago, we designed a light sheet microscope and a chemical clearing to image complete mouse brains. Fluorescence of green fluorescent protein (GFP)-labeled neurons in mouse brains could be preserved with our 3DISCO clearing and high-resolution three-dimensional (3-D) recordings were obtained. Ultramicroscopy was also used to image whole mouse embryos and flies. We improved the optical sectioning of our light sheet microscope by generating longer and thinner light sheets with aspheric optics. To obtain high-resolution images, we corrected available air microscope objectives for clearing solutions with high refractive index. We discuss how eventually super resolution could be realized in light sheet microscopy by applying stimulated emission depletion technology. Also the imaging of brain function by recording of mouse brains expressing cfos-GFP is discussed. Finally, we show the first 3-D recordings of human breast cancer with light sheet microscopy as application in medical diagnostics.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.