ArticleRNA (New York, N.Y.)2016
Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions.
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Terminator-dependent facilitated recycling of RNA polymerase III provides gene-specific transcriptional activation in vivo.Science advances · 2026Article
- Heat stress promotes pre-tRNA capping to modulate cap-dependent translation in Saccharomyces cerevisiae.Nature communications · 2026Article
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- Northern Blotting: Protocols for Radioactive and Nonradioactive Detection of RNA.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Combining Nanopore direct RNA sequencing with genetics and mass spectrometry for analysis of T-loop base modifications across 42 yeast tRNA isoacceptors.Nucleic acids research · 2024Article
- Maf1 phosphorylation is regulated through the action of prefoldin-like Bud27 on PP4 phosphatase in Saccharomyces cerevisiae.Nucleic acids research · 2024Article
- Three tRNA nuclear exporters in S. cerevisiae: parallel pathways, preferences, and precision.Nucleic acids research · 2022Article
- Contribution of domain structure to the function of the yeast DEDD family exoribonuclease and RNase T functional homolog, Rex1.RNA (New York, N.Y.) · 2022Article
- Article
- Review
- Coupling of RNA polymerase III assembly to cell cycle progression in Saccharomyces cerevisiae.Cell cycle (Georgetown, Tex.) · 2019Article
- Function of TFIIIC, RNA polymerase III initiation factor, in activation and repression of tRNA gene transcription.Nucleic acids research · 2018Article
- Signaling to and from the RNA Polymerase III Transcription and Processing Machinery.Annual review of biochemistry · 2018Review
- Archetypal transcriptional blocks underpin yeast gene regulation in response to changes in growth conditions.Scientific reports · 2018Article
- tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- Characterizing Expression and Processing of Precursor and Mature Human tRNAs by Hydro-tRNAseq and PAR-CLIP.Cell reports · 2017Article
- Article
- La Deletion from Mouse Brain Alters Pre-tRNA Metabolism and Accumulation of Pre-5.8S rRNA, with Neuron Death and Reactive Astrocytosis.Molecular and cellular biology · 2017Article
- Transcription by RNA polymerase III: insights into mechanism and regulation.Biochemical Society transactions · 2016Review
- Active Center Control of Termination by RNA Polymerase III and tRNA Gene Transcription Levels In Vivo.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
tRNA is essential for translation and decoding of the proteome. The yeast proteome responds to stress and tRNA biosynthesis contributes in this response by repression of tRNA transcription and alterations of tRNA modification. Here we report that the stress response also involves processing of pre-tRNA 3' termini. By a combination of Northern analyses and RNA sequencing, we show that upon shift to elevated temperatures and/or to glycerol-containing medium, aberrant pre-tRNAs accumulate in yeast cells. For pre-tRNAUAU(Ile) and pre-tRNAUUU Lys) these aberrant forms are unprocessed at the 5' ends, but they possess extended 3' termini. Sequencing analyses showed that partial 3' processing precedes 5' processing for pre-tRNAUAU(Ile). An aberrant pre-tRNA(Tyr) that accumulates also possesses extended 3' termini, but it is processed at the 5' terminus. Similar forms of these aberrant pre-tRNAs are detected in the rex1Δ strain that is defective in 3' exonucleolytic trimming of pre-tRNAs but are absent in the lhp1Δ mutant lacking 3' end protection. We further show direct correlation between the inhibition of 3' end processing rate and the stringency of growth conditions. Moreover, under stress conditions Rex1 nuclease seems to be limiting for 3' end processing, by decreased availability linked to increased protection by Lhp1. Thus, our data document complex 3' processing that is inhibited by stress in a tRNA-type and condition-specific manner. This stress-responsive tRNA 3' end maturation process presumably contributes to fine-tune the levels of functional tRNA in budding yeast in response to environmental conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.