Evidence map›Paper›PMID 26729477›Full record

ArticleArchives of virology2016

Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification.

Jianchang Wang, Libing Liu, Ruiwen Li, Jinfeng Wang, Qi Fu, Wanzhe Yuan

Abstract read
In one paragraph

Article in Archives of virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Real-Time Monitoring on the Chinese Giant Salamander Using RPA-LFD.International journal of molecular sciences · 2024
    Article
  2. Article
  3. Rapid detection ofPeerJ · 2021
    Article
  4. Detection of canine parvovirus and feline panleukopenia virus in fecal samples by strand exchange amplification.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020
    Article
  5. CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2.Chinese chemical letters = Zhongguo hua xue kuai bao · 2019
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. A recombinase polymerase amplification-based assay for rapid detection of African swine fever virus.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2017
    Article
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jianchang WangInspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
Libing LiuInspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
Ruiwen LiCollege of Veterinary Medicine, Agricultural University of Hebei, 38 Lingyusi Street, Baoding, 071001, Hebei, China.
Jinfeng WangInspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
Qi FuInspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau, 318 Heping West Rd, Xinhua District, Shijiazhuang, 050051, Hebei, China.
Wanzhe YuanCollege of Veterinary Medicine, Agricultural University of Hebei, 38 Lingyusi Street, Baoding, 071001, Hebei, China. yuanwanzhe2015@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.

Indexed as

AnimalsDNA, ViralDog DiseasesDogsFecesNucleic Acid Amplification TechniquesParvoviridae InfectionsParvovirus, CaninePolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityTime FactorsDNA, ViralCanine parvovirus type 2Molecular diagnosisPolymerase chain reactionRecombinase polymerase amplification

Identifiers

PMID26729477
PMCPMC7087227

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.