ArticleJournal of the American Society for Mass Spectrometry2016
Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures.
Article in Journal of the American Society for Mass Spectrometry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Nature reviews. Methods primers · 2026Article
- Recent Applications of Artificial Intelligence and Related Technical Challenges in MALDI MS and MALDI-MSI: A Mini Review.Mass spectrometry (Tokyo, Japan) · 2025Review
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- Effect of the Surface Morphology of a Metal Film on Ion Yields in a Platinum-Film Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.Mass spectrometry (Tokyo, Japan) · 2024Article
- The Exploration of Microbial Natural Products and Metabolic Interaction Guided by Mass Spectrometry Imaging.Bioengineering (Basel, Switzerland) · 2022Review
- Spray-Based Application of Matrix to Agar-Based Microbial Samples for Reproducible Sample Adherence in MALDI MSI.Journal of the American Society for Mass Spectrometry · 2022Article
- The application of mass spectrometry imaging in traditional Chinese medicine: a review.Chinese medicine · 2022Review
- Utilizing imaging mass spectrometry to analyze microbial biofilm chemical responses to exogenous compounds.Methods in enzymology · 2022Article
- Factorial Design to Optimize Matrix Spraying Parameters for MALDI Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2021Article
- Expanding Molecular Coverage in Mass Spectrometry Imaging of Microbial Systems Using Metal-Assisted Laser Desorption/Ionization.Microbiology spectrum · 2021Article
- Mass Spectrometry Imaging of Fibroblasts: Promise and Challenge.Expert review of proteomics · 2021Review
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Response Surface Methodology As a New Approach for Finding Optimal MALDI Matrix Spraying Parameters for Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2020Article
- Imaging mass spectrometry for natural products discovery: a review of ionization methods.Natural product reports · 2020Article
- Article
- Quantitative SIMS Imaging of Agar-Based Microbial Communities.Analytical chemistry · 2018Article
- Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria.Journal of the American Society for Mass Spectrometry · 2017Article
- A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms.Journal of mass spectrometry : JMS · 2016Article
- Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking.Nature biotechnology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to visualize biochemical interactions between microbial communities using MALDI MSI has provided tremendous insights into a variety of biological fields. Matrix application using a sieve proved to be incredibly useful, but it has many limitations that include uneven matrix coverage and limitation in the types of matrices that could be employed in studies. Recently, there has been a concerted effort to improve matrix application for studying agar plated microbial cultures, many of which utilized automated matrix sprayers. Here, we describe the usefulness of using a robotic sprayer for matrix application. The robotic sprayer has two-dimensional control over where matrix is applied, and a heated capillary that allows for rapid drying of the applied matrix. This method provided a significant increase in MALDI sensitivity over the sieve method, as demonstrated by FT-ICR MS analysis, facilitating the ability to gain higher lateral resolution MS images of Bacillus subtilis than previously reported. This method also allowed for the use of different matrices to be applied to the culture surfaces.
Indexed as
Identifiers
26729451What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.