ArticleScientific reports2016
Variability of Action Potentials Within and Among Cardiac Cell Clusters Derived from Human Embryonic Stem Cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- In vitro sex-specific function-structure relationship in neonatal rat cardiac monolayers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The importance of matrix in cardiomyogenesis: Defined substrates for maturation and chamber specificity.Matrix biology plus · 2024Article
- Adipocyte-mediated electrophysiological remodeling of human stem cell - derived cardiomyocytes.Journal of molecular and cellular cardiology · 2024Article
- Machine learning-based classification of cardiac relaxation impairment using sarcomere length and intracellular calcium transients.Computers in biology and medicine · 2023Article
- Electrophysiological Properties of Tetraploid Cardiomyocytes Derived from Murine Pluripotent Stem Cells Generated by Fusion of Adult Somatic Cells with Embryonic Stem Cells.International journal of molecular sciences · 2023Article
- Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes.Nature communications · 2022Article
- Assessment of arrhythmia mechanism and burden of the infarcted ventricles following remuscularization with pluripotent stem cell-derived cardiomyocyte patches using patient-derived models.Cardiovascular research · 2022Article
- Computational modeling of aberrant electrical activity following remuscularization with intramyocardially injected pluripotent stem cell-derived cardiomyocytes.Journal of molecular and cellular cardiology · 2022Article
- A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes.Scientific reports · 2021Article
- Machine Learning Techniques to Classify Healthy and Diseased Cardiomyocytes by Contractility Profile.ACS biomaterials science & engineering · 2021Article
- Machine Learning in Arrhythmia and Electrophysiology.Circulation research · 2021Review
- Optical mapping of human embryonic stem cell-derived cardiomyocyte graft electrical activity in injured hearts.Stem cell research & therapy · 2020Article
- DMD Pluripotent Stem Cell Derived Cardiac Cells RecapitulateFrontiers in bioengineering and biotechnology · 2020Article
- Assessing SSRIs' effects on fetal cardiomyocytes utilizing placenta-fetus model.Acta biomaterialia · 2019Article
- Novel method for action potential measurements from intact cardiac monolayers with multiwell microelectrode array technology.Scientific reports · 2019Article
- Contribution of potassium channels to action potential repolarization of human embryonic stem cell-derived cardiomyocytes.British journal of pharmacology · 2019Article
- A comprehensive, multiscale framework for evaluation of arrhythmias arising from cell therapy in the whole post-myocardial infarcted heart.Scientific reports · 2019Article
- Regulation of CaCell calcium · 2019Article
- Representation of Multiple Cellular Phenotypes Within Tissue-Level Simulations of Cardiac Electrophysiology.Bulletin of mathematical biology · 2019Article
- Inversion and computational maturation of drug response using human stem cell derived cardiomyocytes in microphysiological systems.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Electrophysiological variability in cardiomyocytes derived from pluripotent stem cells continues to be an impediment for their scientific and translational applications. We studied the variability of action potentials (APs) recorded from clusters of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) using high-resolution optical mapping. Over 23,000 APs were analyzed through four parameters: APD30, APD80, triangulation and fractional repolarization. Although measures were taken to reduce variability due to cell culture conditions and rate-dependency of APs, we still observed significant variability in APs among and within the clusters. However, similar APs were found in spatial locations with close proximity, and in some clusters formed distinct regions having different AP characteristics that were reflected as separate peaks in the AP parameter distributions, suggesting multiple electrophysiological phenotypes. Using a recently developed automated method to group cells based on their entire AP shape, we identified distinct regions of different phenotypes within single clusters and common phenotypes across different clusters when separating APs into 2 or 3 subpopulations. The systematic analysis of the heterogeneity and potential phenotypes of large populations of hESC-CMs can be used to evaluate strategies to improve the quality of pluripotent stem cell-derived cardiomyocytes for use in diagnostic and therapeutic applications and in drug screening.
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