ArticleBMC genomics2016
Long read and single molecule DNA sequencing simplifies genome assembly and TAL effector gene analysis of Xanthomonas translucens.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Identification and genomic location of resistance to bacterial leaf streak of barley.The plant genome · 2026Article
- Review
- High genomic plasticity and unique features of Xanthomonas translucens pv. graminis revealed through comparative analysis of complete genome sequences.BMC genomics · 2023Article
- Bacterium-enabled transient gene activation by artificial transcription factors for resolving gene regulation in maize.The Plant cell · 2023Article
- Pangenome insights into the diversification and disease specificity of worldwideFrontiers in microbiology · 2023Article
- Characterization of defense responses against bacterial pathogens in duckweeds lacking EDS1.The New phytologist · 2022Article
- Cassava diseases caused by Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae.Molecular plant pathology · 2021Review
- Functional Annotation of a Full-Length Transcriptome and Identification of Genes Associated with Flower Development inPlants (Basel, Switzerland) · 2021Article
- Complete Genome Assemblies of AllFrontiers in microbiology · 2021Article
- Genomics-Enabled Novel Insight Into the Pathovar-Specific Population Structure of the Bacterial Leaf Streak PathogenFrontiers in microbiology · 2021Article
- Factorial estimating assembly base errors usingNAR genomics and bioinformatics · 2020Article
- Single-molecule long-read sequencing of the full-length transcriptome of Rhododendron lapponicum L.Scientific reports · 2020Article
- Identification of a virulence tal gene in the cotton pathogen, Xanthomonas citri pv. malvacearum strain Xss-VBMC microbiology · 2020Article
- The translucens group of Xanthomonas translucens: Complicated and important pathogens causing bacterial leaf streak on cereals.Molecular plant pathology · 2020Article
- Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas.FEMS microbiology reviews · 2020Review
- NovelFrontiers in microbiology · 2020Article
- Analysis of Alternative Splicing and Alternative Polyadenylation inFrontiers in genetics · 2020Article
- Single-molecule real-time sequencing facilitates the analysis of transcripts and splice isoforms of anthers in Chinese cabbage (Brassica rapa L. ssp. pekinensis).BMC plant biology · 2019Article
- Broad-spectrum resistance to bacterial blight in rice using genome editing.Nature biotechnology · 2019Article
- Molecular Typing Reveals High Genetic Diversity of Xanthomonas translucens Strains Infecting Small-Grain Cereals in Iran.Applied and environmental microbiology · 2019Article
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9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe species Xanthomonas translucens encompasses a complex of bacterial strains that cause diseases and yield loss on grass species including important cereal crops. Three pathovars, X. translucens pv. undulosa, X. translucens pv. translucens and X. translucens pv.cerealis, have been described as pathogens of wheat, barley, and oats. However, no complete genome sequence for a strain of this complex is currently available.
resultsA complete genome sequence of X. translucens pv. undulosa strain XT4699 was obtained by using PacBio long read, single molecule, real time (SMRT) DNA sequences and Illumina sequences. Draft genome sequences of nineteen additional X. translucens strains, which were collected from wheat or barley in different regions and at different times, were generated by Illumina sequencing. Phylogenetic relationships among different Xanthomonas strains indicates that X. translucens are members of a distinct clade from so-called group 2 xanthomonads and three pathovars of this species, undulosa, translucens and cerealis, represent distinct subclades in the group 1 clade. Knockout mutation of type III secretion system of XT4699 eliminated the ability to cause water-soaking symptoms on wheat and barley and resulted in a reduction in populations on wheat in comparison to the wild type strain. Sequence comparison of X. translucens strains revealed the genetic variation on type III effector repertories among different pathovars or within one pathovar. The full genome sequence of XT4699 reveals the presence of eight members of the Transcription-Activator Like (TAL) effector genes, which are phylogenetically distant from previous known TAL effector genes of group 2 xanthomonads. Microarray and qRT-PCR analyses revealed TAL effector-specific wheat gene expression modulation.
conclusionsPacBio long read sequencing facilitates the assembly of Xanthomonas genomes and the multiple TAL effector genes, which are difficult to assemble from short read platforms. The complete genome sequence of X. translucens pv. undulosa strain XT4699 and draft genome sequences of nineteen additional X. translucens strains provides a resource for further genetic analyses of pathogenic diversity and host range of the X. translucens species complex. TAL effectors of XT4699 strain play roles in modulating wheat host gene expressions.
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