ArticleCurrent protocols in neuroscience2016
Anterograde or Retrograde Transsynaptic Circuit Tracing in Vertebrates with Vesicular Stomatitis Virus Vectors.
Article in Current protocols in neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Transsynaptic labeling and transcriptional control of zebrafish neural circuits.Nature neuroscience · 2025Article
- Abortive and productive infection of CNS cell types following in vivo delivery of VSV.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Deficiency in the cell-adhesion moleculeFrontiers in cell and developmental biology · 2023Article
- The functional characterization of callosal connections.Progress in neurobiology · 2022Review
- Cre-Dependent Anterograde Transsynaptic Labeling and Functional Imaging in Zebrafish Using VSV With Reduced Cytotoxicity.Frontiers in neuroanatomy · 2021Article
- SARS-CoV-2 and the Nervous System: From Clinical Features to Molecular Mechanisms.International journal of molecular sciences · 2020Review
- Neuroanatomical tract-tracing techniques that did go viral.Brain structure & function · 2020Review
- A mutant vesicular stomatitis virus with reduced cytotoxicity and enhanced anterograde trans-synaptic efficiency.Molecular brain · 2020Article
- Anatomy and Connectivity of the Torus Longitudinalis of the Adult Zebrafish.Frontiers in neural circuits · 2020Article
- Structural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mapping.Frontiers in neural circuits · 2019Article
- Development of an Acute Method to Deliver Transgenes Into the Brains of AdultFrontiers in neural circuits · 2018Article
- Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129.Molecular neurodegeneration · 2017Article
- Chikungunya, Influenza, Nipah, and Semliki Forest Chimeric Viruses with Vesicular Stomatitis Virus: Actions in the Brain.Journal of virology · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells. To date, their use has been largely restricted to mammals. In order to explore the use of such viruses in an expanded host range, we tested the transsynaptic tracing ability of recombinant vesicular stomatitis virus (rVSV) vectors in a variety of organisms. Successful infection and gene expression were achieved in a wide range of organisms, including vertebrate and invertebrate model organisms. Moreover, rVSV enabled transsynaptic tracing of neural circuitry in predictable directions dictated by the viral envelope glycoprotein (G), derived from either VSV or rabies virus (RABV). Anterograde and retrograde labeling, from initial infection and/or viral replication and transmission, was observed in Old and New World monkeys, seahorses, jellyfish, zebrafish, chickens, and mice. These vectors are widely applicable for gene delivery, afferent tract tracing, and/or directional connectivity mapping. Here, we detail the use of these vectors and provide protocols for propagating virus, changing the surface glycoprotein, and infecting multiple organisms using several injection strategies.
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Registered trials
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