ArticleAnalytical chemistry2016
UVliPiD: A UVPD-Based Hierarchical Approach for De Novo Characterization of Lipid A Structures.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Cyclic Peptide-Polymer Conjugate Characterization Using 193 nm Ultraviolet Photodissociation Tandem Mass Spectrometry.Analytical chemistry · 2026Article
- Ganglioside GA2-mediated caspase-11 activation drives macrophage pyroptosis aggravating intimal hyperplasia after arterial injury.International journal of biological sciences · 2025Article
- Identification of a Chimera Mass Spectrum of Isomeric Lipid A Species Using Negative Ion Tandem Mass Spectrometry.Toxins · 2024Article
- Top-Down Characterization of Bacterial Lipopolysaccharides and Lipooligosaccharides Using Activated-Electron Photodetachment Mass Spectrometry.Analytical chemistry · 2024Article
- Structure Determination of Lipid A with Multiple Glycosylation Sites by Tandem MS of Lithium-Adducted Negative Ions.Journal of the American Society for Mass Spectrometry · 2023Article
- LipidA-IDER to Explore the Global Lipid A Repertoire of Drug-Resistant Gram-Negative Bacteria.Analytical chemistry · 2023Article
- Quantitative Analysis and Structural Elucidation of Fatty Acids by Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags andAnalytical chemistry · 2022Article
- Lipid A Structural Determination from a Single Colony.Analytical chemistry · 2022Article
- Study on the CID Fragmentation Pathways of Deprotonated 4'-Monophosphoryl Lipid A.Molecules (Basel, Switzerland) · 2021Article
- Ultraviolet Photodissociation for Non-Target Screening-Based Identification of Organic Micro-Pollutants in Water Samples.Molecules (Basel, Switzerland) · 2020Article
- Article
- Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules.Chemical reviews · 2020Review
- Ion Mobility Spectrometry: Fundamental Concepts, Instrumentation, Applications, and the Road Ahead.Journal of the American Society for Mass Spectrometry · 2019Article
- Top-Down Characterization of Lipooligosaccharides from Antibiotic-Resistant Bacteria.Analytical chemistry · 2019Article
- Characterization of Antigenic Oligosaccharides from Gram-Negative Bacteria via Activated Electron Photodetachment Mass Spectrometry.Analytical chemistry · 2019Article
- Identification of Double Bond Position Isomers in Unsaturated Lipids by m-CPBA Epoxidation and Mass Spectrometry Fragmentation.Analytical chemistry · 2019Article
- Mapping phosphate modifications of substituted lipid A via a targeted MSThe Analyst · 2018Article
- Structural Analysis of Unsaturated Glycosphingolipids Using Shotgun Ozone-Induced Dissociation Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Characterization of Lipid A Variants by Energy-Resolved Mass Spectrometry: Impact of Acyl Chains.Journal of the American Society for Mass Spectrometry · 2017Article
- Progress in Characterization of Lipopolysaccharides and Lipid A by Mass Spectrometry.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The lipid A domain of the endotoxic lipopolysaccharide layer of Gram-negative bacteria is comprised of a diglucosamine backbone to which a variable number of variable length fatty acyl chains are anchored. Traditional characterization of these tails and their linkages by nuclear magnetic resonance (NMR) or mass spectrometry is time-consuming and necessitates databases of pre-existing structures for structural assignment. Here, we introduce an automated de novo approach for characterization of lipid A structures that is completely database-independent. A hierarchical decision-tree MS(n) method is used in conjunction with a hybrid activation technique, UVPDCID, to acquire characteristic fragmentation patterns of lipid A variants from a number of Gram-negative bacteria. Structural assignments are derived from integration of key features from three to five spectra and automated interpretation is achieved in minutes without the need for pre-existing information or candidate structures. The utility of this strategy is demonstrated for a mixture of lipid A structures from an enzymatically modified E. coli lipid A variant. A total of 27 lipid A structures were discovered, many of which were isomeric, showcasing the need for a rapid de novo approach to lipid A characterization.
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