Evidence map›Paper›PMID 26728662›Full record

ArticleJournal of neuroscience research2016

Effects of extracellular calcium and surgical techniques on restoration of axonal continuity by polyethylene glycol fusion following complete cut or crush severance of rat sciatic nerves.

Cameron L Ghergherehchi, George D Bittner, Robert Louis Hastings, Michelle Mikesh, D Colton Riley, Richard C Trevino, Tim Schallert, Wesley P Thayer, Solomon Raju Bhupanapadu Sunkesula, Tu-Anh N Ha and 8 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.

  1. Pooled it
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  13. Facial nerve repair utilizing intraoperative repair strategies.Laryngoscope investigative otolaryngology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 2 institutions in 1 country.

Cameron L GhergherehchiDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
George D BittnerDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Robert Louis HastingsDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Michelle MikeshDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
D Colton RileyDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Richard C TrevinoDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Tim SchallertDepartment of Psychology, University of Texas at Austin, Austin, Texas.
Wesley P ThayerDepartment of Plastic Surgery, Vanderbilt School of Medicine, Nashville, Tennessee.
Solomon Raju Bhupanapadu SunkesulaDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Tu-Anh N HaDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Nicolas MunozDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Monika PyaraliDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Aakarshita BansalDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Andrew D PoonDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Alexander T MazalDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Tyler A SmithDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Nicole S WongDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
Patrick J DunneDepartment of Neuroscience, University of Texas at Austin, Austin, Texas.
The University of Texas at Austin · USVanderbilt University · US

Funding

A novel bioengineered technique to rapidly and permanently repair cut PNS nervesR01NS081063 · NINDS · UNIVERSITY OF TEXAS AT AUSTIN · PI BITTNER, GEORGE DAVIS · 2012 to 2016
$1.7M
NINDS NIH HHS R01 NS081063
6 · The paper itself

Abstract

Complete crush or cut severance of sciatic nerve axons in rats and other mammals produces immediate loss of axonal continuity. Loss of locomotor functions subserved by those axons is restored only after months, if ever, by outgrowths regenerating at ∼1 mm/day from the proximal stumps of severed axonal segments. The distal stump of a severed axon typically begins to degenerate in 1-3 days. We recently developed a polyethylene glycol (PEG) fusion technology, consisting of sequential exposure of severed axonal ends to hypotonic Ca(2+) -free saline, methylene blue, PEG in distilled water, and finally Ca(2+) -containing isotonic saline. This study examines factors that affect the PEG fusion restoration of axonal continuity within minutes, as measured by conduction of action potentials and diffusion of an intracellular fluorescent dye across the lesion site of rat sciatic nerves completely cut or crush severed in the midthigh. Also examined are factors that affect the longer-term PEG fusion restoration of lost behavioral functions within days to weeks, as measured by the sciatic functional index. We report that exposure of cut-severed axonal ends to Ca(2+) -containing saline prior to PEG fusion and stretch/tension of proximal or distal axonal segments of cut-severed axons decrease PEG fusion success. Conversely, trimming cut-severed ends in Ca(2+) -free saline just prior to PEG fusion increases PEG fusion success. PEG fusion prevents or retards the Wallerian degeneration of cut-severed axons, as assessed by measures of axon diameter and G ratio. PEG fusion may produce a paradigm shift in the treatment of peripheral nerve injuries. © 2016 Wiley Periodicals, Inc.

Indexed as

Action PotentialsAnimalsAxonsCalciumDisease Models, AnimalEnzyme InhibitorsFemaleFluorescent DyesMaleMental DisordersNerve RegenerationNeural ConductionNeuromuscular JunctionNeurosurgeryPolyethylene GlycolsRatsCalciumEnzyme InhibitorsFluorescent DyesPolyethylene Glycolsaxonal regenerationaxotomynerve repairpolyethylene glycolrat sciatic nerveWallerian degeneration

Identifiers

PMID26728662
PMCPMC4715960
OpenAlexW2231313877

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.