ArticleBMC microbiology2016
Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 423 citations in OpenAlex.
- WaterborneJournal of water and health · 2024Pooled it
- Protective effect of Glutamicibacter sp. ZY1 against Vibrio parahaemolyticus YDE17 pathogenicity in the clam Ruditapes philippinarum.World journal of microbiology & biotechnology · 2026Article
- Re-examining the iron paradox: the influence of growth medium on regulation and functionality of the tropodithietic acid (TDA) biosynthetic pathway in Phaeobacter piscinae.FEMS microbiology ecology · 2026Article
- Competing Inter-Domain Quorum-Sensing Systems Control Prophage Lysis-Lysogeny Decisions.bioRxiv : the preprint server for biology · 2026Article
- Membrane Vesicle-Mediated Delivery of Antibacterial Lipopeptides byACS chemical biology · 2026Article
- Surface materials and biofilm formation affect tropodithietic acid biosynthesis gene expression inSustainable microbiology · 2026Article
- A comprehensive One Health investigation of erythromycin and quinupristin/dalfopristin resistantJournal of water and health · 2025Article
- Microbial diversity and community structure of microalgae (Nannochloropsis oculata) and rotifer (Brachionus plicatilis) in aquaculture by 16S rRNA amplicon-based sequencing.Antonie van Leeuwenhoek · 2024Article
- The ProbioticMicroorganisms · 2023Article
- Co-Culturing Microalgae withMicroorganisms · 2023Article
- A comprehensive review on human disease-causing bacterial proteases and their impeding agents.Archives of microbiology · 2023Review
- Anaerobic thiosulfate oxidation by the Roseobacter group is prevalent in marine biofilms.Nature communications · 2023Article
- Role is in the eye of the beholder-the multiple functions of the antibacterial compound tropodithietic acid produced by marine Rhodobacteraceae.FEMS microbiology reviews · 2022Article
- Article
- Vibriosis Outbreaks in Aquaculture: Addressing Environmental and Public Health Concerns and Preventive Therapies Using Gilthead Seabream Farming as a Model System.Frontiers in microbiology · 2022Review
- Genomic Evolution of the Marine Bacterium Phaeobacter inhibens during Biofilm Growth.Applied and environmental microbiology · 2021Article
- Contrasting Immunomodulatory Effects of Probiotic and Pathogenic Bacteria on Eastern Oyster,Vaccines · 2020Article
- Bacterial Tropone Natural Products and Derivatives: Overview of their Biosynthesis, Bioactivities, Ecological Role and Biotechnological Potential.Chembiochem : a European journal of chemical biology · 2020Review
- The Probiotic Bacterium Phaeobacter inhibens Downregulates Virulence Factor Transcription in the Shellfish Pathogen Vibrio coralliilyticus byApplied and environmental microbiology · 2019Article
- Bacterial Community Dynamics in an Oyster Hatchery in Response to Probiotic Treatment.Frontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe probiotic bacterium Phaeobacter inhibens strain S4Sm, isolated from the inner shell surface of a healthy oyster, secretes the antibiotic tropodithietic acid (TDA), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. In this study, we investigated the specific roles of TDA secretion and biofilm formation in the probiotic activity of S4Sm.
resultsMutations in clpX (ATP-dependent ATPase) and exoP (an exopolysaccharide biosynthesis gene) were created by insertional mutagenesis using homologous recombination. Mutation of clpX resulted in the loss of TDA production, no decline in biofilm formation, and loss of the ability to inhibit the growth of Vibrio tubiashii and Vibrio anguillarum in co-colonization experiments. Mutation of exoP resulted in a ~60% decline in biofilm formation, no decline in TDA production, and delayed inhibitory activity towards Vibrio pathogens in co-colonization experiments. Both clpX and exoP mutants exhibited reduced ability to protect oyster larvae from death when challenged by Vibrio tubiashii. Complementation of the clpX and exoP mutations restored the wild type phenotype. We also found that pre-colonization of surfaces by S4Sm was critical for this bacterium to inhibit pathogen colonization and growth.
conclusionsOur observations demonstrate that probiotic activity by P. inhibens S4Sm involves contributions from both biofilm formation and the production of the antibiotic TDA. Further, probiotic activity also requires colonization of surfaces by S4Sm prior to the introduction of the pathogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.