ArticleAnalytical chemistry2016
Microfabrication and in Vivo Performance of a Microdialysis Probe with Embedded Membrane.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 71 citations in OpenAlex.
- Local Chemical Gradients in a Mammalian Cortex Measured In Vivo With a Silicon Nanodialysis Mass Spectrometry Platform.Angewandte Chemie (International ed. in English) · 2026Article
- Region-specific proteomic profiling of brain interstitial fluidLab on a chip · 2026Article
- Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.ACS measurement science au · 2025Article
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- Enhancement of faradaic current in an electrochemical cell integrated into silicon microfluidic channels.Sensors and actuators. B, Chemical · 2023Article
- Customizable, wireless and implantable neural probe design and fabrication via 3D printing.Nature protocols · 2023Review
- Letting the little light of mind shine: Advances and future directions in neurochemical detection.Neuroscience research · 2022Review
- Small molecules released from islets of Langerhans determined by liquid chromatography - mass spectrometry.Analytical methods : advancing methods and applications · 2022Article
- Wireless, battery-free push-pull microsystem for membrane-free neurochemical sampling in freely moving animals.Science advances · 2022Article
- Microdialysis and microperfusion electrodes in neurologic disease monitoring.Fluids and barriers of the CNS · 2021Review
- Methods for intratumoral microdialysis probe targeting and validation in murine brain tumor models.Journal of neuroscience methods · 2021Article
- HIV-Associated Apathy/Depression and Neurocognitive Impairments Reflect Persistent Dopamine Deficits.Cells · 2021Review
- Fiber-Based Electrochemical Biosensors for Monitoring pH and Transient Neurometabolic Lactate.Analytical chemistry · 2021Article
- A 3D flexible neural interface based on a microfluidic interconnection cable capable of chemical delivery.Microsystems & nanoengineering · 2021Article
- Platform for micro-invasive membrane-free biochemical sampling of brain interstitial fluid.Science advances · 2020Article
- Article
- Imaging Guidance for Therapeutic Delivery: The Dawn of Neuroenergetics.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2020Review
- Microneedles for transdermal diagnostics: Recent advances and new horizons.Biomaterials · 2020Review
- Metabolic profiling of dialysate at sensitized acupoints in knee osteoarthritis patients: A study protocol.Medicine · 2019Article
- Next-generation interfaces for studying neural function.Nature biotechnology · 2019Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Microdialysis sampling is an essential tool for in vivo neurochemical monitoring. Conventional dialysis probes are over 220 μm in diameter and have limited flexibility in design because they are made by assembly using preformed membranes. The probe size constrains spatial resolution and governs the amount of tissue damaged caused by probe insertion. To overcome these limitations, we have developed a method to microfabricate probes in Si that are 45 μm thick × 180 μm wide. The probes contain a buried, U-shaped channel that is 30 μm deep × 60 μm wide and terminates in ports for external connection. A 4 mm length of the probe is covered with a 5 μm thick nanoporous membrane. The membrane was microfabricated by deep reactive ion etching through a porous aluminum oxide layer. The microfabricated probe has cross-sectional area that is 79% less than that of the smallest conventional microdialysis probes. The probes yield 2-20% relative recovery at 100 nL/min perfusion rate for a variety of small molecules. The probe was successfully tested in vivo by sampling from the striatum of live rats. Fractions were collected at 20 min intervals (2 μL) before and after an intraperitoneal injection of 5 mg/kg amphetamine. Analysis of fractions by liquid chromatography-mass spectrometry revealed reliable detection of 14 neurochemicals, including dopamine and acetylcholine, at basal conditions. Amphetamine evoked a 43-fold rise in dopamine, a result nearly identical to a conventional dialysis probe in the same animal. The microfabricated probes have potential for sampling with higher spatial resolution and less tissue disruption than conventional probes. It may also be possible to add functionality to the probes by integrating other components, such as electrodes, optics, and additional channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.