ArticleScientific reports2016
Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Infection cycles of viruses of the phylum Nucleocytoviricota.Nature reviews. Microbiology · 2026Review
- Decapod iridescent virus 1 (DIV1) enters hematopoieticJournal of virology · 2026Article
- Characterizing Marine Medaka (Animals : an open access journal from MDPI · 2024Article
- A Collagen10a1 mutation disrupts cell polarity in a medaka model for metaphyseal chondrodysplasia type Schmid.iScience · 2024Article
- Near-atomic architecture of Singapore grouper iridovirus and implications for giant virus assembly.Nature communications · 2023Article
- Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication.International journal of molecular sciences · 2021Article
- Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.PLoS pathogens · 2020Article
- Co-Expression Network Analysis of Spleen Transcriptome in Rock Bream (International journal of molecular sciences · 2020Article
- Virus capsid assembly across different length scales inspire the development of virus-based biomaterials.Current opinion in virology · 2019Review
- Description of a Natural Infection with Decapod Iridescent Virus 1 in Farmed Giant Freshwater Prawn,Viruses · 2019Article
- Assessment of fish iridoviruses using a novel cell line GS-1, derived from the spleen of orange-spotted grouper Epinephelus coioides (Hamilton) and susceptible to ranavirus and megalocytivirus.The Journal of veterinary medical science · 2018Article
- Review
- Subcellular redistribution and sequential recruitment of macromolecular components during SGIV assembly.Protein & cell · 2016Article
- Recombinant Ranaviruses for Studying Evolution of Host-Pathogen Interactions in Ectothermic Vertebrates.Viruses · 2016Review
- Endoplasmic Reticulum: The Favorite Intracellular Niche for Viral Replication and Assembly.Viruses · 2016Review
- Organelle dynamics and viral infections: at cross roads.Microbes and infectionReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iridovirid infection is associated with the catastrophic loss in aquaculture industry and the population decline of wild amphibians and reptiles, but none of the iridovirid life cycles have been well explored. Here, we report the detailed visualization of the life cycle of Singapore grouper iridovirus (SGIV) in grouper cells by cryo-electron microscopy (cryoEM) and tomography (ET). EM imaging revealed that SGIV viral particles have an outer capsid layer, and the interaction of this layer with cellular plasma membrane initiates viral entry. Subsequent viral replication leads to formation of a viral assembly site (VAS), where membranous structures emerge as precursors to recruit capsid proteins to form an intermediate, double-shell, crescent-shaped structure, which curves to form icosahedral capsids. Knockdown of the major capsid protein eliminates the formation of viral capsids. As capsid formation progresses, electron-dense materials known to be involved in DNA encapsidation accumulate within the capsid until it is fully occupied. Besides the well-known budding mechanism through the cell periphery, we demonstrate a novel budding process in which viral particles bud into a tubular-like structure within vacuoles. This budding process may denote a new strategy used by SGIV to disseminate viral particles into neighbor cells while evading host immune response.
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