ArticlePLoS neglected tropical diseases2016
The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Updated annotation and meta-analysis of Brugia malayi transcriptomics data reveals consistent transcriptional profiles across time and space with some study-specific differences in adult female worm transcriptional profiles.PLoS neglected tropical diseases · 2024Pooled it
- Gene regulation in response to host sex and infection route in Brugia pahangi with new genome annotation.G3 (Bethesda, Md.) · 2026Article
- Striatal transcriptomic alterations immediately after short-term abstinence from methamphetamine self-administration in rats.Molecular brain · 2025Article
- Pharmacological Profiling of a Brugia malayi Muscarinic Acetylcholine Receptor as a Putative Antiparasitic Target.Antimicrobial agents and chemotherapy · 2023Article
- Dirofilariasis mouse models for heartworm preclinical research.Frontiers in microbiology · 2023Article
- A mouse infection model and long-term lymphatic endothelium co-culture system to evaluate drugs against adult Brugia malayi.PLoS neglected tropical diseases · 2022Article
- Comparative analysis on transcriptomics of ivermectin resistant and susceptible strains of Haemonchus contortus.Parasites & vectors · 2022Article
- Spatial transcriptomics reveals antiparasitic targets associated with essential behaviors in the human parasite Brugia malayi.PLoS pathogens · 2022Article
- Phylum-Spanning Neuropeptide GPCR Identification and Prioritization: Shaping Drug Target Discovery Pipelines for Nematode Parasite Control.Frontiers in endocrinology · 2021Article
- Genetic and functional diversification of chemosensory pathway receptors in mosquito-borne filarial nematodes.PLoS biology · 2020Article
- Comparative analysis of small RNAs released by the filarial nematode Litomosoides sigmodontis in vitro and in vivo.PLoS neglected tropical diseases · 2019Article
- Effects of diethylcarbamazine and ivermectin treatment onParasitology open · 2019Article
- Genetic Crosses and Linkage Mapping in Schistosome Parasites.Trends in parasitology · 2018Review
- Of dogs and hookworms: man's best friend and his parasites as a model for translational biomedical research.Parasites & vectors · 2018Review
- Mining Filarial Genomes for Diagnostic and Therapeutic Targets.Trends in parasitology · 2018Review
- WormBase ParaSite - a comprehensive resource for helminth genomics.Molecular and biochemical parasitology · 2017Article
- Susceptibility Testing of Medically Important Parasites.Clinical microbiology reviews · 2017Review
- The role of 'omics' in the quest to eliminate human filariasis.PLoS neglected tropical diseases · 2017Review
- Direct experimental manipulation of intestinal cells in Ascaris suum, with minor influences on the global transcriptome.International journal for parasitology · 2017Article
- Profiling the macrofilaricidal effects of flubendazole on adult female Brugia malayi using RNAseq.International journal for parasitology. Drugs and drug resistance · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFilarial nematodes cause serious and debilitating infections in human populations of tropical countries, contributing to an entrenched cycle of poverty. Only one human filarial parasite, Brugia malayi, can be maintained in rodents in the laboratory setting. It has been a widely used model organism in experiments that employ culture systems, the impact of which on the worms is unknown. METHODOLOGY/PRINCIPAL
findingsUsing Illumina RNA sequencing, we characterized changes in gene expression upon in vitro maintenance of adult B. malayi female worms at four time points: immediately upon removal from the host, immediately after receipt following shipment, and after 48 h and 5 days in liquid culture media. The dramatic environmental change and the 24 h time lapse between removal from the host and establishment in culture caused a globally dysregulated gene expression profile. We found a maximum of 562 differentially expressed genes based on pairwise comparison between time points. After an initial shock upon removal from the host and shipping, a few stress fingerprints remained after 48 h in culture and until the experiment was stopped. This was best illustrated by a strong and persistent up-regulation of several genes encoding cuticle collagens, as well as serpins. CONCLUSIONS/SIGNIFICANCE: These findings suggest that B. malayi can be maintained in culture as a valid system for pharmacological and biological studies, at least for several days after removal from the host and adaptation to the new environment. However, genes encoding several stress indicators remained dysregulated until the experiment was stopped.
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