ArticlePloS one2016
Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Engineered Phytoene-Producing Saccharomyces cerevisiae Alleviates Cyclophosphamide-Induced Intestinal Barrier Dysfunction and Gut Dysbiosis.Probiotics and antimicrobial proteins · 2026Article
- Cross-comparison of strains used for mitochondrial imaging inLife science alliance · 2025Article
- Genetically encoded protein oscillators for FM streaming of single-cell data.bioRxiv : the preprint server for biology · 2025Article
- Shining a light on the impact of antifungals onAntimicrobial agents and chemotherapy · 2024Article
- Article
- Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa.Fungal genetics and biology : FG & B · 2023Article
- Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone.Communications chemistry · 2022Article
- Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Global analysis of Saccharomyces cerevisiae growth in mucin.G3 (Bethesda, Md.) · 2021Article
- Article
- Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors.Frontiers in microbiology · 2021Article
- Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light-Activated Guide RNA.Angewandte Chemie (International ed. in English) · 2020Article
- FRET Microscopy in Yeast.Biosensors · 2019Review
- Imaging and analysis of genetically encoded calcium indicators linking neural circuits and behaviors.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2019Review
- Analysis of the roles of phosphatidylinositol-4,5-Molecular biology of the cell · 2019Article
- Fluorescent indicators for simultaneous reporting of all four cell cycle phases.Nature methods · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. However, the availability of optimal fluorescent protein combinations for 3-color imaging is limited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue dyes, such as DAPI, for potential four-color live cell imaging.
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Registered trials
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