ArticlePLoS genetics2016
TrpA1 Regulates Defecation of Food-Borne Pathogens under the Control of the Duox Pathway.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 84 citations in OpenAlex.
- Uracil-driven ROS signaling and larval TrpA1-B neuron activation are required for priming the adult Drosophila gustatory response to bacteria.PLoS biology · 2026Article
- A pore-forming toxin monalysin contributes to infection-induced suppression of defecation in female Drosophila.PLoS pathogens · 2026Article
- Sex differences inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Drosophila melanogaster mitigates gastro-oral infections by stimulating pathogen expulsion.BMC microbiology · 2026Article
- Diacylglycerol metabolism drives host-pathogen responses during enteric infection inbioRxiv : the preprint server for biology · 2025Article
- Evolution of resistance and disease tolerance mechanisms to oral bacterial infection inOpen biology · 2025Article
- Article
- Molecular Functions and Physiological Roles of Gustatory Receptors of the SilkwormInternational journal of molecular sciences · 2024Review
- Ingestion of Bacillus cereus spores dampens the immune response to favor bacterial persistence.Nature communications · 2024Article
- Pathobiont and symbiont contribute to microbiota homeostasis through Malpighian tubules-gut countercurrent flow in Bactrocera dorsalis.The ISME journal · 2024Article
- Infection risk by oral contamination does not induce immune priming in the mealworm beetle (Frontiers in immunology · 2024Article
- Bioelectric regulation of intestinal stem cells.Trends in cell biology · 2023Review
- TrpA1 is a shear stress mechanosensing channel regulating intestinal stem cell proliferation inScience advances · 2023Article
- Gut AstA mediates sleep deprivation-induced energy wasting in Drosophila.Cell discovery · 2023Article
- Stilbenoid compounds inhibit NF-κB-mediated inflammatory responses in theFrontiers in immunology · 2023Article
- Population bottlenecks constrain host microbiome diversity and genetic variation impeding fitness.PLoS genetics · 2022Article
- Nociception and hypersensitivity involve distinct neurons and molecular transducers inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Dorsal switch protein 1 as a damage signal in insect gut immunity to activate dual oxidaseFrontiers in immunology · 2022Article
- Glutamate metabolism directs energetic trade-offs to shape host-pathogen susceptibility in Drosophila.Cell metabolism · 2021Article
- Activation ofInternational journal of molecular sciences · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogen expulsion from the gut is an important defense strategy against infection, but little is known about how interaction between the intestinal microbiome and host immunity modulates defecation. In Drosophila melanogaster, dual oxidase (Duox) kills pathogenic microbes by generating the microbicidal reactive oxygen species (ROS), hypochlorous acid (HOCl) in response to bacterially excreted uracil. The physiological function of enzymatically generated HOCl in the gut is, however, unknown aside from its anti-microbial activity. Drosophila TRPA1 is an evolutionarily conserved receptor for reactive chemicals like HOCl, but a role for this molecule in mediating responses to gut microbial content has not been described. Here we identify a molecular mechanism through which bacteria-produced uracil facilitates pathogen-clearing defecation. Ingestion of uracil increases defecation frequency, requiring the Duox pathway and TrpA1. The TrpA1(A) transcript spliced with exon10b (TrpA1(A)10b) that is present in a subset of midgut enteroendocrine cells (EECs) is critical for uracil-dependent defecation. TRPA1(A)10b heterologously expressed in Xenopus oocytes is an excellent HOCl receptor characterized with elevated sensitivity and fast activation kinetics of macroscopic HOCl-evoked currents compared to those of the alternative TRPA1(A)10a isoform. Consistent with TrpA1's role in defecation, uracil-excreting Erwinia carotovora showed higher persistence in TrpA1-deficient guts. Taken together, our results propose that the uracil/Duox pathway promotes bacteria expulsion from the gut through the HOCl-sensitive receptor, TRPA1(A)10b, thereby minimizing the chances that bacteria adapt to survive host defense systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.