ArticleScientific reports2016
Amy63, a novel type of marine bacterial multifunctional enzyme possessing amylase, agarase and carrageenase activities.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 50 citations in OpenAlex.
- Genomic and Phenotypic Analysis of Antarctic Marine Cryobacterium sp. PAMC25264.Current microbiology · 2026Article
- Bioconversion of macroalgae by enzymes from associated fungi into hydrolysates with cumulative antioxidant and antiglycemic properties for effective diabetes management.Archives of microbiology · 2026Article
- Advances in Carrageenases: Molecular Engineering, Immobilization Techniques, and Biomedical Potential of Carrageenan Oligosaccharides.Marine drugs · 2026Review
- Genomic insights into carbohydrate-active enzymes in a novel Radiobacillus strain from the Antarctic red macroalga Pyropia endiviifolia.World journal of microbiology & biotechnology · 2026Article
- Discovery and engineering of bifunctional enzymes for lignocellulose degradation: Metagenomic and computational approaches.Biotechnology reports (Amsterdam, Netherlands) · 2025Review
- Light and polyphosphate kinase 2 cooperatively regulate the production of zero-valent sulfur in a deep-sea bacterium.mSystems · 2025Article
- Whole genome sequencing of a novel carrageenan-degrading bacteriumFrontiers in microbiology · 2025Article
- The Effects of Agaro-Oligosaccharides Produced by Marine Bacteria (Marine drugs · 2024Article
- Molecular identification of Halomonas AZ07 and its multifunctional enzymatic activities to degrade Pyropia yezoensis under high-temperature condition.Molecular biology reports · 2024Article
- Exploring a novel GH13_5 α-amylase from Jeotgalibacillus malaysiensis D5AMB Express · 2024Article
- Herbivorous Fish Microbiome Adaptations to Sulfated Dietary Polysaccharides.Applied and environmental microbiology · 2023Article
- Assessment ofRevista de biologia tropical · 2023Article
- Comparative analysis of genome-based CAZyme cassette in Antarctic Microbacterium sp. PAMC28756 with 31 other Microbacterium species.Genes & genomics · 2022Article
- Activity-Based Screening of Soil Samples from Nyingchi, Tibet, for Amylase-Producing Bacteria and Other Multifunctional Enzyme Capacities.International journal of microbiology · 2022Article
- Multifunctional alkalophilic α-amylase with diverse raw seaweed degrading activities.AMB Express · 2021Article
- A CAZyme-Rich Genome of a Taxonomically Novel Rhodophyte-Associated Carrageenolytic Marine Bacterium.Marine biotechnology (New York, N.Y.) · 2018Article
- Draft Genome Sequence of Newly Isolated Agarolytic Bacteria Cellulophaga omnivescoria sp. nov. W5C Carrying Several Gene Loci for Marine Polysaccharide Degradation.Current microbiology · 2018Article
- Bacterial carrageenases: an overview of production and biotechnological applications.3 Biotech · 2016Review
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multifunctional enzyme is one that performs multiple physiological functions, thus benefiting the organism. Characterization of multifunctional enzymes is important for researchers to understand how organisms adapt to different environmental challenges. In the present study, we report the discovery of a novel multifunctional enzyme Amy63 produced by marine bacterium Vibrio alginolyticus 63. Remarkably, Amy63 possesses amylase, agarase and carrageenase activities. Amy63 is a substrate promiscuous α-amylase, with the substrate priority order of starch, carrageenan and agar. Amy63 maintains considerable amylase, carrageenase and agarase activities and stabilities at wide temperature and pH ranges, and optimum activities are detected at temperature of 60 °C and pH of 6.0, respectively. Moreover, the heteroexpression of Amy63 dramatically enhances the ability of E. coli to degrade starch, carrageenan and agar. Motif searching shows three continuous glycosyl hydrolase 70 (GH70) family homologs existed in Amy63 encoding sequence. Combining serial deletions and phylogenetic analysis of Amy63, the GH70 homologs are proposed as the determinants of enzyme promiscuity. Notably, such enzymes exist in all kingdoms of life, thus providing an expanded perspective on studies of multifunctional enzymes. To our knowledge, this is the first report of an amylase having additional agarase and carrageenase activities.
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