Evidence map›Paper›PMID 26725115›Full record

ArticleCell reports2016

Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue.

Zahra Erami, David Herrmann, Sean C Warren, Max Nobis, Ewan J McGhee, Morghan C Lucas, Wilfred Leung, Nadine Reischmann, Agata Mrowinska, Juliane P Schwarz and 24 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 87 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022
    Article
  13. Review
  14. Article
  15. Review
  16. Arf6 determines tissue architecture by stabilizing intercellular adhesion.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020
    Article
  17. Article
  18. Article
  19. Review
  20. Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

34 authors at 6 institutions in 2 countries.

Zahra EramiCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
David HerrmannThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Sean C WarrenThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Max NobisCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Ewan J McGheeCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Morghan C LucasThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Wilfred LeungThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Nadine ReischmannThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Agata MrowinskaCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Juliane P SchwarzCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Shereen KadirCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
James R W ConwayThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Claire VenninThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Saadia A KarimCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Andrew D CampbellCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
David Gallego-OrtegaThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Astrid MagenauThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Kendelle J MurphyThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Rachel A RidgwayCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Andrew M LawThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Stacey N WaltersThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Shane T GreyThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
David R CroucherThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Lei ZhangThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Herbert HerzogThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
Edna C HardemanNeuromuscular and Regenerative Medicine Unit, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Peter W GunningOncology Research Unit, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Christopher J OrmandyThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia.
T R Jeffry EvansCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Douglas StrathdeeCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Owen J SansomCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Jennifer P MortonCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Kurt I AndersonCancer Research UK Beatson Institute, Switchback Road, Bearsden, Glasgow G61 1BD, UK. Electronic address: k.anderson@beatson.gla.ac.uk.
Paul TimpsonThe Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW 2010, Australia. Electronic address: p.timpson@garvan.org.au.
Cancer Research UK · GBCancer Research UK Scotland Institute · GBThe Kinghorn Cancer Centre · AUGarvan Institute of Medical Research · AUSt Vincent's Clinic · AUUNSW Sydney · AU

Funding

Cancer Research UK 11650Cancer Research UK 12946Cancer Research UK 15565Cancer Research UK 20409Cancer Research UK 21139
6 · The paper itself

Abstract

E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments.

Indexed as

Tumor MicroenvironmentAnimalsCadherinsGreen Fluorescent ProteinsMiceMice, TransgenicNeoplasms, ExperimentalOptical ImagingOrgan SpecificityProto-Oncogene Proteins p21(ras)Tumor Suppressor Protein p53CadherinsGreen Fluorescent ProteinsHras protein, mouseProto-Oncogene Proteins p21(ras)Tumor Suppressor Protein p53cell adhesion and migrationE-cadherinFRAPintravital imaginginvasion and metastasisKrasp53pancreatic cancerSrc-kinase

Identifiers

PMID26725115
PMCPMC4709331
OpenAlexW2255577075

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.