Evidence map›Paper›PMID 26724953›Full record

ArticleExtremophiles : life under extreme conditions2016

Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid.

Manel Ben Abdallah, Fatma Karray, Najla Mhiri, Nan Mei, Marianne Quéméneur, Jean-Luc Cayol, Gaël Erauso, Jean-Luc Tholozan, Didier Alazard, Sami Sayadi

Abstract read
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In one paragraph

Article in Extremophiles : life under extreme conditions, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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  7. Production of Poly(3-Hydroxybutyrate) byArchaea (Vancouver, B.C.) · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Manel Ben AbdallahLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.
Fatma KarrayLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia. fatma.karray@cbs.rnrt.tn.ORCID http://orcid.org/0000-0002-7390-2270
Najla MhiriLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.
Nan MeiIRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France.
Marianne QuéméneurIRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France.
Jean-Luc CayolIRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France.
Gaël ErausoIRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France.
Jean-Luc TholozanIRD, University of Aix-Marseille, University of Toulon, CNRS, MIO, UM 110, 13288, Marseille Cedex 09, France.
Didier AlazardLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.
Sami SayadiLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018, Sfax, Tunisia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 × 10(6) DNA copies g(-1) whereas archaea varied between 5 × 10(5) and 10(6) DNA copies g(-1) in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid.

Indexed as

MicrobiotaSalinitySalt ToleranceArchaeaBacillotaDeinococcusLakesProteobacteriaRNA, Ribosomal, 16SThermusTunisiaRNA, Ribosomal, 16S16S rRNAAnaerobic fermentative bacteriaArchaeaBacteriaHypersaline lakeMicrobial diversityPCR-DGGEQuantitative PCR

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.