ArticleCurrent protocols in human genetics2016
GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos.
Article in Current protocols in human genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- CRISPR-based genome editing of a diurnal rodent, Nile grass rat (Arvicanthis niloticus).BMC biology · 2024Article
- Embryo and fetal gene editing: Technical challenges and progress toward clinical applications.Molecular therapy. Methods & clinical development · 2024Review
- Single-cell synaptome mapping: its technical basis and applications in critical period plasticity research.Frontiers in neural circuits · 2024Review
- Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice.Genome biology · 2022Article
- Gender-Difference in Hair Length as Revealed by Crispr-Based Production of Long-Haired Mice with Dysfunctional FGF5 Mutations.International journal of molecular sciences · 2022Article
- From genotype to phenotype: genetics of mammalian long non-coding RNAs in vivo.Nature reviews. Genetics · 2022Review
- GONAD: A new method for germline genome editing in mice and rats.Development, growth & differentiation · 2021Article
- Designing and generating a mouse model: frequently asked questions.Journal of biomedical research · 2021Article
- Generation of mouse conditional knockout alleles in one step using theGenome research · 2021Article
- Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models.Frontiers in genetics · 2021Review
- Review
- Extracellular Vesicles Function as Bioactive Molecular Transmitters in the Mammalian Oviduct: An Inspiration for Optimizing in Vitro Culture Systems and Improving Delivery of Exogenous Nucleic Acids during Preimplantation Embryonic Development.International journal of molecular sciences · 2020Review
- Genome editing methods in animal models.Animal cells and systems · 2020Review
- Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD.Nature protocols · 2019Article
- Generating mouse models for biomedical research: technological advances.Disease models & mechanisms · 2019Review
- i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats.Scientific reports · 2018Article
- Successful production of genome-edited rats by the rGONAD method.BMC biotechnology · 2018Article
- i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.Genome biology · 2018Article
- Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses.Nature communications · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Transgenic technologies used for creating a desired genomic change in animals involve three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them and their subsequent transfer to recipient females. These ex vivo steps have been widely used for over 3 decades and they were also readily adapted for the latest genome editing technologies such as ZFNs, TALENs, and CRISPR/Cas9 systems. We recently developed a method called GONAD (Genome editing via Oviductal Nucleic Acids Delivery) that does not require all the three critical steps of transgenesis and therefore relieves the bottlenecks of widely used animal transgenic technologies. Here we provide protocols for the GONAD system.
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Registered trials
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