Evidence map›Paper›PMID 26724083›Full record

ArticleThe Review of scientific instruments2015

Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging.

M S DeVore, D G Stich, A M Keller, C Cleyrat, M E Phipps, J A Hollingsworth, D S Lidke, B S Wilson, P M Goodwin, J H Werner

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Review of scientific instruments, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023
    Review
  2. Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

M S DeVoreCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
D G StichCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
A M KellerCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
C CleyratDepartment of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
M E PhippsCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
J A HollingsworthCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
D S LidkeDepartment of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
B S WilsonDepartment of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
P M GoodwinCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
J H WernerCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.

Funding

UNM TRAININGP50GM085273 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI EDWARDS, JEREMY S · 2009 to 2018
$26.1M
Mapping for Specialized Domains for FCeRI Signaling & InternalizationR01AI051575 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI WILSON, BRIDGET S · 2003 to 2013
$3.3M
Three-Dimensional Molecular Tracking of IgE-Fc{epsilon}RI in Live CellsR01AI097154 · NIAID · LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB · PI WERNER, JAMES H · 2012 to 2015
$1.6M
Single Molecule Imaging to Quantify FcεRI Signaling DynamicsR01GM100114 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI LIDKE, DIANE · 2012 to 2016
$1.4M
Functional Analysis of the Sulfotransferase, SULT2B1F32GM065794 · NIGMS · INSTITUTE FOR CANCER RESEARCH · PI GEESE, WILLIAM J · 2002 to 2002
$31k
NIAID NIH HHS 5R01AI097154NIAID NIH HHS R01 AI051575NIAID NIH HHS R01AI051575NIAID NIH HHS R01 AI097154NIGMS NIH HHS F32 GM065794NIGMS NIH HHS P50 GM065794NIGMS NIH HHS P50GM0852673NIGMS NIH HHS P50 GM085273NIGMS NIH HHS R01 GM100114NIGMS NIH HHS R01GM100114
6 · The paper itself

Abstract

We describe recent upgrades to a 3D tracking microscope to include simultaneous Nipkow spinning disk imaging and time-gated single-particle tracking (SPT). Simultaneous 3D molecular tracking and spinning disk imaging enable the visualization of cellular structures and proteins around a given fluorescently labeled target molecule. The addition of photon time-gating to the SPT hardware improves signal to noise by discriminating against Raman scattering and short-lived fluorescence. In contrast to camera-based SPT, single-photon arrival times are recorded, enabling time-resolved spectroscopy (e.g., measurement of fluorescence lifetimes and photon correlations) to be performed during single molecule/particle tracking experiments.

Indexed as

Quantum DotsCell TrackingEquipment DesignEquipment Failure AnalysisImage EnhancementImaging, Three-DimensionalMicroscopy, FluorescenceMolecular ImagingReproducibility of ResultsSensitivity and SpecificitySignal Processing, Computer-Assisted

Identifiers

PMID26724083
PMCPMC4676784

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.