Evidence map›Paper›PMID 26723877›Full record

ArticleCancer letters2016

Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1.

Hyang Sook Seol, Sang Eun Lee, Joon Seon Song, Je-Keun Rhee, Shree Ram Singh, Suhwan Chang, Se Jin Jang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 74 citations in OpenAlex.

  1. Systemic and local dysregulation of the complement system in glioma: a comprehensive systematic review.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Hyang Sook SeolAsan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
Sang Eun LeeAsan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
Joon Seon SongDepartment of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
Je-Keun RheeDepartment of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
Shree Ram SinghBasic Research Laboratory, Stem Cell Regulation and Animal Aging Section, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
Suhwan ChangDepartment of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: suhwan.chang@amc.seoul.kr.
Se Jin JangAsan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: jangsejin@amc.seoul.kr.
Ulsan College · KRNational Cancer Institute · US

Funding

Intramural NIH HHS Z99 CA999999
6 · The paper itself

Abstract

Tumor-initiating cells are important for the formation and maintenance of tumor bulks in various tumors. To identify surface markers of liver tumor-initiating cells, we performed primary tumorsphere culture and analyzed the expression of cluster of differentiation (CD) antigen genes using NanoString. Interestingly, we found significant upregulation of the complement proteins (p = 1.60 × 10(-18)), including C7 and CFH. Further studies revealed that C7 and CFH are required to maintain stemness in liver cancer cells. Knockdown of C7 and CFH expression abrogated tumorsphere formation and induced differentiation, whereas overexpression stimulated stemness factor expression as well as in vivo cell growth. Mechanistically, by studying C7 and CFH-dependent LSF-1 expression and its direct role on stemness factor transcription, we found that LSF-1 is involved in this regulation. Taken together, our data demonstrate the unprecedented role of complement proteins on the maintenance of stemness in liver tumor-initiating cells.

Indexed as

Cell DifferentiationCell ProliferationAdultAgedAnimalsCell Line, TumorComplement C7Complement Factor HDNA-Binding ProteinsFemaleGene Expression Regulation, NeoplasticHeterograftsHumansImmunity, InnateLiver NeoplasmsMaleCFH protein, humanComplement C7Complement Factor HDNA-Binding ProteinsTFCP2 protein, humanTranscription FactorsCFHComplement protein C7HCCLiver tumor-initiating cellLSF-1Stemness factor

Identifiers

PMID26723877
PMCPMC4744505
OpenAlexW2198350064

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.