ArticleInternational journal of clinical and experimental pathology2015
Effect of hypoxia on the expression of RANKL/OPG in human periodontal ligament cells in vitro.
Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- TRPV1-linked neuropeptide axes in periodontitis and peri-implantitis.Frontiers in endocrinology · 2026Review
- Association of Salivary HIF-1α Level With Epithelial and Mesenchymal Markers in Smokers and Nonsmokers With Periodontitis: A Case-Control Study.International journal of dentistry · 2026Article
- Vascularized in vitro bone model as 3D quadruple culture with primary human osteoblasts, osteocytes, osteoclasts and endothelial cells.Materials today. Bio · 2025Article
- Association of Polymorphism with Periodontitis and Salivary Levels of Hypoxia-Inducible Factor-1α.European journal of dentistry · 2025Article
- Review
- Ozone therapy in dentistry: An overview of the biological mechanisms involved (Review).Biomedical reports · 2024Review
- Exploring the Expression of Pro-Inflammatory and Hypoxia-Related MicroRNA-20a, MicroRNA-30e, and MicroRNA-93 in Periodontitis and Gingival Mesenchymal Stem Cells under Hypoxia.International journal of molecular sciences · 2022Article
- Article
- Hyperbaric Oxygen Therapy Does Not Have a Negative Impact on Bone Signaling Pathways in Humans.Healthcare (Basel, Switzerland) · 2021Article
- Association of high HIF-1α levels in serous periodontitis with external root resorption by the NFATc1 pathway.Journal of molecular histology · 2020Article
- Selection and validation of reference genes by RT-qPCR for murine cementoblasts in mechanical loading experiments simulating orthodontic forces in vitro.Scientific reports · 2020Article
- High levels of HIF-1ɑ in hypoxic dental pulps associated with teeth with severe periodontitis.Journal of molecular histology · 2020Article
- Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio.BMC oral health · 2020Article
- Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats.Journal of applied oral science : revista FOB · 2020Article
- Protective effect and related mechanisms of curcumin in rat experimental periodontitis.Head & face medicine · 2018Article
- Transcriptional activation of glucose transporter 1 in orthodontic tooth movement-associated mechanical response.International journal of oral science · 2018Article
- Article
- Osteoprotegerin Gene (OPG) Polymorphisms Associated with Peri-implantitis Susceptibility in a Chinese Han Population.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- RANK-RANKL-OPG expression after gingival mesenchymal stem cell hypoxia preconditioned application in an orthodontic tooth movement animal model.Journal of oral biology and craniofacial researchArticle
- Relationship between soluble neuropilin-1 in the gingival crevicular fluid of early pregnant women and different severities of periodontitis: A cross-sectional study.Journal of oral biology and craniofacial researchArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the impact of hypoxia on the expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in human periodontal ligament cells (hPDLCs) in vitro. hPDLCs were incubated in a hypoxic atmosphere of 2% O2, 5% CO2, 94% N2 at 37°C for 6, 12, 24 and 48 h. After that, cell proliferation assay was determined using CCK-8 technique. SP immunocytochemistry method was performed to trace the expression of hypoxia-inducible factor 1 alpha (HIF-1α) in hPDLCs. The expression levels of RANKL and OPG were investigated using real-time PCR and ELISA. As a control, the cells were incubated at normoxic conditions of 20% O2, 5% CO2, 75% N2. All results were analyzed using one-way ANOVA at a significant level of P=0.05. OPG mRNA and protein levels were down-regulated meanwhile RANKL mRNA and soluble RANKL (sRANKL) protein levels were up-regulated after stimulated by hypoxia. The relative RANKL/OPG expression ratios were increased in both mRNA and protein levels. The expression of RANKL mRNA and sRANKL protein levels was enhanced significantly (P<0.05) under the hypoxia conditions at 12 h, 24 h and 48 h while OPG mRNA and protein were reduced significantly (P<0.05) at 12 h, 24 h and 48 h. Hypoxia can affect the expression of RANKL and OPG in hPDLCs, which constitute an important pathogenic event in the alveolar bone resorption. Lack of oxygen in periodontal tissue may accelerate the development of periodontitis.
Indexed as
Identifiers
26722486PMC4680431W2408937785What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.