ArticleInternational journal of biological sciences2016
Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4.
Article in International journal of biological sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- In Vitro Characterization of Reversine-Treated Gingival Fibroblasts and Their Safety Evaluation after In Vivo Transplantation.Pharmaceutics · 2024Article
- Miniature-swine iPSC-derived GABA progenitor cells function in a rat Parkinson's disease model.Cell and tissue research · 2023Article
- Reversine ameliorates hallmarks of cellular senescence in human skeletal myoblasts via reactivation of autophagy.Aging cell · 2023Article
- [Directed differentiation of human induced pluripotent stem cells into midbrain].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023Article
- Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease.International journal of molecular medicine · 2022Article
- Reversine enhances the conversion of dedifferentiated fat cells into mature cardiomyocytes.F1000Research · 2022Article
- RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF‑β1/Smad pathway.International journal of molecular medicine · 2021Article
- [Directed differentiation of porcine induced pluripotent stem cells into forebrain GABAergic neuron progenitors].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Multiple functions of reversine on the biological characteristics of sheep fibroblasts.Scientific reports · 2021Article
- Effects of Different Concentrations of Reversine on Plasticity of Mesenchymal Stem Cells.Indian journal of clinical biochemistry : IJCB · 2020Article
- Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts.Scientific reports · 2019Article
- Relation ofCancer cell international · 2019Article
- Reversine inhibits Colon Carcinoma Cell Migration by Targeting JNK1.Scientific reports · 2018Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reversine, a purine analog, had been evidenced that it could induce dedifferentiation of differentiated cells into multipotent progenitor cells. Here, we showed that reversine could increase the plasticity of long-term cryopreserved bovine fibroblasts, and reversine-treated cells achieved the ability to differentiate into all three germ layers cells, such as osteoblasts and adipocytes from mesoblast, neurocyte from ectoderm, hepatocytes and smooth muscle cells from endoderm. Moreover, treatment of reversine caused the grow arrest of fibroblasts at G2/M and distinct cell swelling resulting in the formation of polyploid cells. In parallel, reversine treatment induced a multipotency of fibroblasts might be attributed to the activation of histone modifications, especially the degression of DNA methylation. However, molecular and cellular experiments suggested that reversine treatment enhanced selectively the expression of pluripotent marker gene Oct4 and mesenchymal marker genes CD29, CD44 and CD73, but Sox2 and Nanog were not detected. Taken together, these results clearly demonstrate the ability of reversine to dedifferentiation of long-term cryopreserved somatic cells through activation of pluripotent gene Oct4.
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