Evidence map›Paper›PMID 26721831›Full record

ArticleCurrent microbiology2016

Novel Development of a qPCR Assay Based on the rpoB Gene for Rapid Detection of Cronobacter spp.

Yuanhong Li, Qiming Chen, Hua Jiang, Yang Jiao, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu

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Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. DecipheringMicroorganisms · 2026
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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yuanhong LiCollege of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Jiangsu, 210095, Nanjing, People's Republic of China.
Qiming ChenCollege of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Jiangsu, 210095, Nanjing, People's Republic of China.
Hua JiangCollege of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Jiangsu, 210095, Nanjing, People's Republic of China.
Yang JiaoSchool of Public Health, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, 221004, Jiangsu, People's Republic of China.
Fengxia LuCollege of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Jiangsu, 210095, Nanjing, People's Republic of China.
Xiaomei BieCollege of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Jiangsu, 210095, Nanjing, People's Republic of China.
Zhaoxin LuCollege of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Jiangsu, 210095, Nanjing, People's Republic of China. fmb@njau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel real-time PCR (qPCR) assay with internal amplification control based on the rpoB gene was developed for the detection and quantification of Cronobacter spp. Inclusivity and exclusivity of the qPCR assay were tested on a strain collection containing 19 Cronobacter and 26 non-Cronobacter strains. All Cronobacter strains were successfully identified, whereas no cross-reactivity was observed with non-Cronobacter strains. The sensitivity of the qPCR assay for pure culture and powdered infant formula (PIF) without enrichment was 3.44 log CFU/ml(g) (2.74 × 10(3) CFU/ml(g)). When the qPCR assay was applied to artificially contaminated PIF after a 12-h enrichment step, as few as 0.03 log CFU/ml (1.06 × 10(0) CFU/ml) of C. sakazakii could be detected. The limit of detection of the qPCR assay was not reduced by the presence of 8 log CFU/ml of Salmonella Enteritidis in PIF. A total of 70 food samples were analyzed for the presence of Cronobacter spp., out of which 3 dry cereal products, 5 maternal milk, and 1 infant food formula were found as positive by qPCR. The results obtained by qPCR were consistent with those obtained by culture-based method. Results from this study demonstrate that the qPCR assay is a rapid, specific, and accurate method suitable for Cronobacter detection in foods.

Indexed as

Genes, BacterialReal-Time Polymerase Chain ReactionCronobacterFood ContaminationFood MicrobiologyHumansSensitivity and Specificity

Identifiers

PMID26721831

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