Evidence map›Paper›PMID 26721447›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2016

Spatiotemporal release of BMP-2 and VEGF enhances osteogenic and vasculogenic differentiation of human mesenchymal stem cells and endothelial colony-forming cells co-encapsulated in a patterned hydrogel.

Danial Barati, Seyed Ramin Pajoum Shariati, Seyedsina Moeinzadeh, Juan M Melero-Martin, Ali Khademhosseini, Esmaiel Jabbari

Expression of concernOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 67 papers.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed
8.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed, 153 citations in OpenAlex.

  1. Review
  2. Molecular-level understanding of the aging bone and regeneration mechanisms using computational methods.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2026
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7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Danial BaratiBiomimetic Materials and Tissue Engineering Laboratory, Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.
Seyed Ramin Pajoum ShariatiBiomimetic Materials and Tissue Engineering Laboratory, Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.
Seyedsina MoeinzadehBiomimetic Materials and Tissue Engineering Laboratory, Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.
Juan M Melero-MartinDepartment of Cardiac Surgery, Boston Children's Hospital, Boston, MA 02115, USA.
Ali KhademhosseiniBiomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston 02139, MA, USA; Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, MA, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston 02115, MA, USA; Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Hwayangdong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Esmaiel JabbariBiomimetic Materials and Tissue Engineering Laboratory, Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA. Electronic address: jabbari@mailbox.sc.edu.
University of South Carolina · USBoston Children's Hospital · USBrigham and Women's Hospital · US

Funding

Vascular niche bioengineering for human bone regenerationR01AR069038 · NIAMS · BOSTON CHILDREN'S HOSPITAL · PI MELERO-MARTIN, JUAN M · 2016 to 2020
$1.9M
Microengineered Osteoinductive and Vasculogenic ScaffoldR56AR063745 · NIAMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI JABBARI, ESMAIEL · 2013 to 2014
$669k
NIAMS NIH HHS AR063745NIAMS NIH HHS R01 AR069038NIAMS NIH HHS R56 AR063745
6 · The paper itself

Abstract

Reconstruction of large bone defects is limited by insufficient vascularization and slow bone regeneration. The objective of this work was to investigate the effect of spatial and temporal release of recombinant human bone morphogenetic protein-2 (BMP2) and vascular endothelial growth factor (VEGF) on the extent of osteogenic and vasculogenic differentiation of human mesenchymal stem cells (hMSCs) and endothelial colony-forming cells (ECFCs) encapsulated in a patterned hydrogel. Nanogels (NGs) based on polyethylene glycol (PEG) macromers chain-extended with short lactide (L) and glycolide (G) segments were used for grafting and timed-release of BMP2 and VEGF. NGs with 12kDa PEG molecular weight (MW), 24 LG segment length, and 60/40L/G ratio (P12-II, NG(10)) released the grafted VEGF in 10days. NGs with 8kDa PEG MW, 26 LG segment length, and 60/40L/G ratio (P8-I, NG(21)) released the grafted BMP2 in 21days. hMSCs and NG-BMP2 were encapsulated in a patterned matrix based on acrylate-functionalized lactide-chain-extended star polyethylene glycol (SPELA) hydrogel and microchannel patterns filled with a suspension of hMSCs+ECFCs and NG-VEGF in a crosslinked gelatin methacryloyl (GelMA) hydrogel. Groups included patterned constructs without BMP2/VEGF (None), with directly added BMP2/VEGF, and NG-BMP2/NG-VEGF. Based on the results, timed-release of VEGF in the microchannels in 10days from NG(10) and BMP2 in the matrix in 21days from NG(21) resulted in highest extent of osteogenic and vasculogenic differentiation of the encapsulated hMSCs and ECFCs compared to direct addition of VEGF and BMP2. Further, timed-release of VEGF from NG(10) in hMSC+ECFC encapsulating microchannels and BMP2 from NG(21) in hMSC encapsulating matrix sharply increased bFGF expression in the patterned constructs. The results suggest that mineralization and vascularization are coupled by localized secretion of paracrine signaling factors by the differentiating hMSCs and ECFCs.

Indexed as

Antigens, CDBone Morphogenetic Protein 2Cadherin 5CadherinsCell DifferentiationCells, CulturedCore Binding Factor Alpha 1 SubunitDNAEndothelium, VascularHumansHydrogelsNeovascularization, PhysiologicOsteogenesisPlatelet Endothelial Cell Adhesion Molecule-1PolyestersPolyethylene GlycolsAntigens, CDBone Morphogenetic Protein 2Cadherin 5CadherinsCore Binding Factor Alpha 1 SubunitDNAHydrogelsPlatelet Endothelial Cell Adhesion Molecule-1PolyestersPolyethylene GlycolsPolyglycolic Acidpoly(lactide)recombinant human bone morphogenetic protein-2Recombinant ProteinsRNA, MessengerRUNX2 protein, humanTransforming Growth Factor betaVascular Endothelial Growth Factor AVEGFA protein, humanDual protein deliveryECFCHuman MSCNanogel graftingOsteogenesisPatterned hydrogelrhBMP-2VasculogenesisVEGF

Identifiers

PMID26721447
PMCPMC4724464
OpenAlexW2282869468

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.