ArticlePLoS neglected tropical diseases2015
Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Test accuracy of loop-mediated isothermal amplification for schistosomiasis in low endemicity areas: a systematic review and meta-analysis.Infectious diseases of poverty · 2025Pooled it
- Towards point-of-care diagnostics of neglected tropical diseases using nucleic acid amplification tests.Analytical and bioanalytical chemistry · 2026Review
- Schistosomiasis diagnosis: Challenges and opportunities for elimination.PLoS neglected tropical diseases · 2024Review
- Article
- Single- and Two-Stage, Closed-Tube, Point-of-Care, Molecular Detection of SARS-CoV-2.Analytical chemistry · 2021Article
- LAMP in Neglected Tropical Diseases: A Focus on Parasites.Diagnostics (Basel, Switzerland) · 2021Review
- Loop-Mediated Isothermal Amplification in Schistosomiasis.Journal of clinical medicine · 2021Review
- Comparison of quantitative polymerase chain reaction, Kato-Katz and circulating cathodic antigen rapid test for the diagnosis ofCurrent research in parasitology & vector-borne diseases · 2021Article
- Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics.Micromachines · 2020Review
- Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics.Nucleic acids research · 2020Article
- Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.Infectious diseases of poverty · 2019Review
- A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples.PLoS neglected tropical diseases · 2018Article
- A Multifunctional Reactor with Dry-Stored Reagents for Enzymatic Amplification of Nucleic Acids.Analytical chemistry · 2018Article
- Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection.Clinical chemistry · 2017Article
- Miniaturized devices for point of care molecular detection of HIV.Lab on a chip · 2017Review
- Point-of-Care Diagnostics: Recent Developments in a Connected Age.Analytical chemistry · 2017Review
- Biompha-LAMP: A New Rapid Loop-Mediated Isothermal Amplification Assay for Detecting Schistosoma mansoni in Biomphalaria glabrata Snail Host.PLoS neglected tropical diseases · 2016Article
- Instrument-Free Point-of-Care Molecular Detection of Zika Virus.Analytical chemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Parasitic helminths such as schistosomes, as well as filarial and soil-transmitted nematodes, are estimated to infect at least a billion people worldwide, with devastating impacts on human health and economic development. Diagnosis and monitoring of infection dynamics and efficacy of treatment depend almost entirely on methods that are inaccurate, labor-intensive, and unreliable. These shortcomings are amplified and take on added significance in mass drug administration programs, where measures of effectiveness depend on accurate monitoring of treatment success (or failure), changes in disease transmission rates, and emergence of possible drug resistance. Here, we adapt isothermal molecular assays such as loop-mediated isothermal amplification (LAMP) to a simple, hand-held, custom-made field-ready microfluidic device that allows sensitive and specific detection of schistosome cell-free nucleic acids in serum and plasma (separated with a point-of-care plasma separator) from Schistosoma mansoni-infected mice. Cell-free S. mansoni DNA was detected with our device without prior extraction from blood. Our chip exhibits high sensitivity (~2 x 10(-17) g/μL), with a positive signal for S. mansoni DNA detectable as early as one week post infection, several weeks before parasite egg production commences. These results indicate that incorporation of isothermal amplification strategies with our chips could represent a strategy for rapid, simple, low-cost diagnosis of both pre-patent and chronic schistosome infections as well as potential monitoring of treatment efficacy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.