ArticleInvestigative ophthalmology & visual science2015
Physical Factors Affecting Outflow Facility Measurements in Mice.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 38 citations in OpenAlex.
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- Aging and intraocular pressure homeostasis in mice.Aging cell · 2024Article
- Effect of a fixed combination of ripasudil and brimonidine on aqueous humor dynamics in mice.Scientific reports · 2024Article
- A Histomorphometric and Computational Investigation of the Stabilizing Role of Pectinate Ligaments in the Aqueous Outflow Pathway.bioRxiv : the preprint server for biology · 2024Article
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- Intracameral Injection of AAV-DJ.COMP-ANG1 Reduces the IOP of Mice by Reshaping the Trabecular Outflow Pathway.Investigative ophthalmology & visual science · 2022Article
- Measurement of postmortem outflow facility using iPerfusion.Experimental eye research · 2022Article
- Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.Investigative ophthalmology & visual science · 2022Review
- Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri).Investigative ophthalmology & visual science · 2022Article
- Effects of acute stress, general anesthetics, tonometry, and temperature on intraocular pressure in rats.Experimental eye research · 2021Article
- Surface Engineering of FLT4-Targeted Nanocarriers Enhances Cell-Softening Glaucoma Therapy.ACS applied materials & interfaces · 2021Article
- The ocular pulse decreases aqueous humor outflow resistance by stimulating nitric oxide production.American journal of physiology. Cell physiology · 2021Article
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- In vivo estimation of murine iris stiffness using finite element modeling.Experimental eye research · 2021Article
- Aqueous Humor Outflow Requires Active Cellular Metabolism in Mice.Investigative ophthalmology & visual science · 2020Article
- Integral role for lysyl oxidase-like-1 in conventional outflow tissue function and behavior.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- The β4-Subunit of the Large-Conductance Potassium Ion Channel KCa1.1 Regulates Outflow Facility in Mice.Investigative ophthalmology & visual science · 2020Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
purposeMice are commonly used to study conventional outflow physiology. This study examined how physical factors (hydration, temperature, and anterior chamber [AC] deepening) influence ocular perfusion measurements in mice.
methodsOutflow facility (C) and pressure-independent outflow (Fu) were assessed by multilevel constant pressure perfusion of enucleated eyes from C57BL/6 mice. To examine the effect of hydration, seven eyes were perfused at room temperature, either immersed to the limbus in saline and covered with wet tissue paper or exposed to room air. Temperature effects were examined in 12 eyes immersed in saline at 20 °C or 35 °C. Anterior chamber deepening was examined in 10 eyes with the cannula tip placed in the anterior versus posterior chamber (PC). Posterior bowing of the iris (AC deepening) was visualized by three-dimensional histology in perfusion-fixed C57BL/6 eyes and by spectral-domain optical coherence tomography in living CD1 mice.
resultsExposure to room air did not significantly affect C, but led to a nonzero Fu that was significantly reduced upon immersion in saline. Increasing temperature from 20 °C to 35 °C increased C by 2.5-fold, more than could be explained by viscosity changes alone (1.4-fold). Perfusion via the AC, but not the PC, led to posterior iris bowing and increased outflow.
conclusionsInsufficient hydration contributes to the appearance of pressure-independent outflow in enucleated mouse eyes. Despite the large lens, AC deepening may artifactually increase outflow in mice. Temperature-dependent metabolic processes appear to influence conventional outflow regulation. Physical factors should be carefully controlled in any outflow studies involving mice.
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