ArticleInvestigative ophthalmology & visual science2015
Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Research on interdisciplinary measurement and evolutionary path in the field of ophthalmology.Frontiers in ophthalmology · 2025Article
- Demonstration of the pathogenicity of a common non-exomic mutation in ABCA4 using iPSC-derived retinal organoids and retrospective clinical data.Human molecular genetics · 2024Article
- Establishment of an in vitro choroid complex system for vascular response screening.Scientific reports · 2024Article
- Propensity of Patient-Derived iPSCs for Retinal Differentiation: Implications for Autologous Cell Replacement.Stem cells translational medicine · 2023Article
- Does senescence play a role in age-related macular degeneration?Experimental eye research · 2022Review
- Single-cell transcriptomic landscapes of a rare human laryngeal chondrosarcoma.Journal of cancer research and clinical oncology · 2022Article
- The vascular niche in next generation microphysiological systems.Lab on a chip · 2021Review
- Single-cell RNA sequencing in vision research: Insights into human retinal health and disease.Progress in retinal and eye research · 2021Review
- Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids.International journal of molecular sciences · 2021Article
- Stepwise differentiation and functional characterization of human induced pluripotent stem cell-derived choroidal endothelial cells.Stem cell research & therapy · 2020Article
- Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid.The Journal of experimental medicine · 2020Article
- Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell-Based Treatments.Stem cells translational medicine · 2019Article
- Choriocapillaris Degeneration in Geographic Atrophy.The American journal of pathology · 2019Article
- Sorsby fundus dystrophy: Insights from the past and looking to the future.Journal of neuroscience research · 2019Review
- CRISPR-Cas9 genome engineering: Treating inherited retinal degeneration.Progress in retinal and eye research · 2018Review
- Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells.Biology open · 2018Article
- Cellular regeneration strategies for macular degeneration: past, present and future.Eye (London, England) · 2018Review
- Identification of a synergistic interaction between endothelial cells and retinal pigment epithelium.Journal of cellular and molecular medicine · 2017Article
- Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy.Current protocols in stem cell biology · 2017Article
- Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies.Acta biomaterialia · 2017Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
purposeAge-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells currently are a major scientific focus. However, the success of these approaches likely also will require replacement of the underlying, supportive choroidal vasculature. The purpose of this study was to generate stem cell-derived CECs to develop efficient differentiation and transplantation protocols.
methodsDermal fibroblasts from the Tie2-GFP mouse were isolated and reprogrammed into two independent induced pluripotent stem cell (iPSC) lines via viral transduction of the transcription factors Oct4, Sox2, Klf4, and c-Myc. Tie2-GFP iPSCs were differentiated into CECs using a coculture method with either the RF6A CEC line or primary mouse CECs. Induced pluripotent stem cell-derived CECs were characterized via RT-PCR and immunocytochemistry for EC- and CEC-specific markers.
resultsInduced pluripotent stem cells generated from mice expressing green fluorescent protein (GFP) under control of the endothelial Tie2 promoter display classic pluripotency markers and stem cell morphology. Induced pluripotent stem cell-derived CECs express carbonic anhydrase IV, eNOS, FOXA2, PLVAP, CD31, CD34, ICAM-1, Tie2, TTR, VE-cadherin, and vWF.
conclusionsInduced pluripotent stem cell-derived CECs will be a valuable tool for modeling of choriocapillaris-specific insults in AMD and for use in future choroidal endothelial cell replacement approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.