ArticleJournal of biomedical optics2016
D38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage.
Article in Journal of biomedical optics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 93 citations in OpenAlex.
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Spectroscopic Active Probes for Investigation of Lipid Transformation in Cells and Membranes.The journal of physical chemistry. B · 2026Article
- Raman imaging of molecular groups in the wavenumber silent region.Analytical and bioanalytical chemistry · 2026Review
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Nuclear envelope-associated lipid droplets are enriched in cholesteryl esters and increase during inflammatory signaling.The EMBO journal · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Machine learning empowered coherent Raman imaging and analysis for biomedical applications.Communications engineering · 2025Review
- An Activity-Based Sensing Approach to Multiplex Mapping of Labile Copper Pools by Stimulated Raman Scattering.Journal of the American Chemical Society · 2024Article
- Fast Real-Time Brain Tumor Detection Based on Stimulated Raman Histology and Self-Supervised Deep Learning Model.Journal of imaging informatics in medicine · 2024Article
- Vibrational imaging of metabolites for improved microbial cell strains.Journal of biomedical optics · 2024Review
- Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level.ACS central science · 2024Review
- Biomedical applications, perspectives and tag design concepts in the cell - silent Raman window.RSC chemical biology · 2024Review
- Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control.Nature communications · 2024Article
- A Raman spectral marker for the iso-octyl chain structure of cholesterol.Analytical science advances · 2024Article
- Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases.International journal of molecular sciences · 2023Review
- Bringing Vibrational Imaging to Chemical Biology with Molecular Probes.ACS chemical biology · 2022Article
- Dissecting lipid droplet biology with coherent Raman scattering microscopy.Journal of cell science · 2022Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
We generated a highly deuterated cholesterol analog (D38-cholesterol) and demonstrated its use for selective vibrational imaging of cholesterol storage in mammalian cells. D38-cholesterol produces detectable signals in stimulated Raman scattering (SRS) imaging, is rapidly taken up by cells, and is efficiently metabolized by acyl-CoA cholesterol acyltransferase to form cholesteryl esters. Using hyperspectral SRS imaging of D38-cholesterol, we visualized cholesterol storage in lipid droplets. We found that some lipid droplets accumulated preferentially unesterified D38-cholesterol, whereas others stored D38-cholesteryl esters. In steroidogenic cells, D38-cholesteryl esters and triacylglycerols were partitioned into distinct sets of lipid droplets. Thus, hyperspectral SRS imaging of D38-cholesterol demonstrates a heterogeneous incorporation of neutral lipid species, i.e., free cholesterol, cholesteryl esters, and triacylglycerols, between individual lipid droplets in a cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.