ArticleBiotechnology for biofuels2015
The impact of a single-nucleotide mutation of bgl2 on cellulase induction in a Trichoderma reesei mutant.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 62 citations in OpenAlex.
- Advances in microbial biofuel production by metabolic and enzyme engineering, synthetic biology, metagenomics, and genome editing applications.Emerging topics in life sciences · 2025Review
- Involvement of the intracellular β-glucosidase BGL1B from Aspergillus niger in the regulation of lignocellulose-degrading enzymes' synthesis.Biotechnology for biofuels and bioproducts · 2025Article
- Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production inJournal of fungi (Basel, Switzerland) · 2023Article
- A microfluidic device for simultaneous detection of enzyme secretion and elongation of a single hypha.Frontiers in microbiology · 2023Article
- Inducer-free cellulase production system based on the constitutive expression of mutated XYR1 and ACE3 in the industrial fungus Trichoderma reesei.Scientific reports · 2022Article
- Taxonomy, comparative genomics and evolutionary insights of Penicillium ucsense: a novel species in series Oxalica.Antonie van Leeuwenhoek · 2022Article
- From induction to secretion: a complicated route for cellulase production in Trichoderma reesei.Bioresources and bioprocessing · 2021Review
- Article
- Involvement of Xyr1 and Are1 for Trichodermapepsin Gene Expression in Response to Cellulose and Galactose in Trichoderma reesei.Current microbiology · 2020Article
- Studies of Cellulose and Starch Utilization and the Regulatory Mechanisms of Related Enzymes in Fungi.Polymers · 2020Review
- Diversity of Cellulase-Producing Filamentous Fungi From Tibet and Transcriptomic Analysis of a Superior Cellulase ProducerFrontiers in microbiology · 2020Article
- Engineering ofBiotechnology for biofuels · 2020Article
- Harnessing the knowledge of protein secretion for enhanced protein production in filamentous fungi.World journal of microbiology & biotechnology · 2019Review
- The putative β-glucosidase BGL3I regulates cellulase induction inBiotechnology for biofuels · 2018Article
- Self-induction system for cellulase production by cellobiose produced from glucose in Rhizopus stolonifer.Scientific reports · 2017Article
- A novel GH10 xylanase fromBiotechnology for biofuels · 2017Article
- Genome sequencing and transcriptome analysis ofBiotechnology for biofuels · 2017Article
- A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production.Microbial cell factories · 2016Article
- Genome wide analysis of protein production load in Trichoderma reesei.Biotechnology for biofuels · 2016Article
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina) produces increased cellulase expression when grown on cellulose or its derivatives as a sole carbon source. It has been believed that β-glucosidases of T. reesei not only metabolize cellobiose but also contribute in the production of inducers of cellulase gene expression by their transglycosylation activity. The cellulase hyper-producing mutant PC-3-7 developed in Japan has enhanced cellulase production ability when cellobiose is used as the inducer. The comparative genomics analysis of PC-3-7 and its parent revealed a single-nucleotide mutation within the bgl2 gene encoding intracellular β-glucosidase II (BGLII/Cel1a), giving rise to an amino acid substitution in PC-3-7, which could potentially account for the enhanced cellulase expression when these strains are cultivated on cellulose and cellobiose.
resultsTo analyze the effects of the BGLII mutation in cellulase induction, we constructed both a bgl2 revertant and a disruptant. Enzymatic analysis of the transformant lysates showed that the strain expressing mutant BGLII exhibited weakened cellobiose hydrolytic activity, but produced some transglycosylation products, suggesting that the SNP in bgl2 strongly diminished cellobiase activity, but did not result in complete loss of function of BGLII. The analysis of the recombinant BGLII revealed that transglycosylation products might be oligosaccharides, composed probably of glucose linked β-1,4, β-1,3, or a mixture of both. PC-3-7 revertants of bgl2 exhibited reduced expression and inducibility of cellulase during growth on cellulose and cellobiose substrates. Furthermore, the effect of this bgl2 mutation was reproduced in the common strain QM9414 in which the transformants showed cellulase production comparable to that of PC-3-7.
conclusionWe conclude that BGLII plays an important role in cellulase induction in T. reesei and that the bgl2 mutation in PC-3-7 brought about enhanced cellulase expression on cellobiose. The results of the investigation using PC-3-7 suggested that other mutation(s) in PC-3-7 could also contribute to cellulase induction. Further investigation is essential to unravel the mechanism responsible for cellulase induction in T. reesei.
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