ArticleClinical chemistry2016
Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays.
Article in Clinical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed.
- Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics.Proteomics · 2026Article
- Discovery and Validation of Novel Umami Peptides from Traditional Broad Bean Paste (Doubanjiang).Foods (Basel, Switzerland) · 2026Article
- Proteomic approaches for interrogating kinase signaling networks.The Journal of investigative dermatology · 2026Article
- Hemoglobin alpha-like subunit mu is expressed during ontogeny and is elevated in alpha thalassemia.Blood red cells & iron · 2026Article
- IsoPS-DIA: Dual Functionality of Absolute Targeted Quantification and Global Proteome Profiling.Analytical chemistry · 2026Article
- The GF-NEO discovery platform unveils a [KQE][DG] sequence motif within fusion neoantigens in pediatric cancer.iScience · 2026Article
- Screening for Peptides to Bind and Functionally Inhibit SARS-CoV-2 Fusion Peptide Using Mirrored Combinatorial Phage Display and Human Proteomic Phage Display.Molecules (Basel, Switzerland) · 2026Article
- Blood Matrices and Sample Preparation Influence Blood Marker Discovery.Journal of proteome research · 2026Article
- Advances in Multiple Reaction Monitoring (MRM) for Targeted Proteomics in Biomedical Research.Advances in experimental medicine and biology · 2026Review
- The Detrimental Effects of Crystalline Excipients: How They Jeopardize the Long-Term Stability of Freeze-Dried Polypeptide Formulations.Pharmaceutics · 2025Article
- Pre-analytical drivers of bias in bead-enriched plasma proteomics.EMBO molecular medicine · 2025Article
- A standardized framework for circulating blood proteomics.Nature genetics · 2025Review
- Quantification of parathyroid hormone in high-purity materials by two isotope dilution mass spectrometry methods.Analytical and bioanalytical chemistry · 2025Article
- Quantification of glucagon and oxyntomodulin by protein precipitation-immunoaffinity enrichment-LC-MS/MS.Journal of mass spectrometry and advances in the clinical lab · 2025Article
- iDIA-QC: AI-empowered data-independent acquisition mass spectrometry-based quality control.Nature communications · 2025Article
- Article
- A Streamlined High-Throughput LC-MS Assay for Quantifying Peptide Degradation in Cell Culture.Journal of biomedical materials research. Part A · 2025Article
- A Streamlined High-Throughput LC-MS Assay for Quantifying Peptide Degradation in Cell Culture.bioRxiv : the preprint server for biology · 2024Article
- Characterization of an expanded set of assays for immunomodulatory proteins using targeted mass spectrometry.Scientific data · 2024Article
- Sterol Derivatives Specifically Increase Anti-Inflammatory Oxylipin Formation in M2-like Macrophages by LXR-Mediated Induction of 15-LOX.Molecules (Basel, Switzerland) · 2024Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
55 authors.
Funding
Abstract
backgroundFor many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope-labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays. CONTENT: The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials-in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry-is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care.
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Registered trials
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