ArticleJournal of clinical microbiology2016
In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Antimicrobial prescribing guidelines for horses in Australia.Australian veterinary journal · 2025Guideline
- The Apparent pH Stability of Antibiotic Preparations Used for Uterine Infusions in Mares.Animals : an open access journal from MDPI · 2026Article
- Antibiofilm action of ozonated sunflower oil against bacteria isolated from the uterus of mares susceptible to endometritis.Animal reproduction · 2026Article
- Newly isolated bacteriophages show efficacy and phage-antibiotic synergy in vitro against the equine genital pathogens Klebsiella pneumoniae and Pseudomonas aeruginosa.BMC veterinary research · 2025Article
- In vitro antimicrobial activity of non-traditional therapies for infectious endometritis in mares.Equine veterinary journal · 2025Article
- Fundamentals of microbiome-based therapies for reproductive tract inflammatory diseases in domestic animals.Animal reproduction · 2025Review
- Adjunctive intravesical EDTA-tromethamine treatment of a biofilm-associated recurrentThe Canadian veterinary journal = La revue veterinaire canadienne · 2024Article
- Platelet-Rich Plasma Proteome of Mares Susceptible to Persistent-Breeding-Induced Endometritis Differs from Resistant Mares.Animals : an open access journal from MDPI · 2024Article
- Untargeted Metabolomic Analysis Reveals Plasma Differences between Mares with Endometritis and Healthy Ones.Animals : an open access journal from MDPI · 2024Article
- Isolation and characterization of bacteriophages specific toFrontiers in microbiology · 2024Article
- Effect of Sampling Method on Detection of the Equine Uterine Microbiome during Estrus.Veterinary sciences · 2023Article
- Genotypic and Phenotypic Characterization ofAnimals : an open access journal from MDPI · 2023Article
- The Role of Biofilms in the Pathogenesis of Animal Bacterial Infections.Microorganisms · 2023Review
- Antimicrobial Susceptibility of Bacterial Isolates from Donkey Uterine Infections, 2018-2021.Veterinary sciences · 2022Article
- Complete Genome Sequences of Eight Streptococcus equi subsp.Microbiology resource announcements · 2021Article
- A Novel Approach to Minimising Acute Equine Endometritis That May Help to Prevent the Development of the Chronic State.Frontiers in veterinary science · 2021Article
- Fungistatic Action of N-Acetylcysteine onMicroorganisms · 2020Article
- Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology.International journal of molecular sciences · 2020Review
- Bacterial Biofilm and its Role in the Pathogenesis of Disease.Antibiotics (Basel, Switzerland) · 2020Review
- Complete Genome Sequence of Pandoraea pnomenusa TF-18, a Multidrug-Resistant Organism Isolated from the Rhizosphere of Rice (Oryza sativa L. subsp.Microbiology resource announcements · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we evaluated the ability of the equine clinical treatments N-acetylcysteine, EDTA, and hydrogen peroxide to disrupt in vitro biofilms and kill equine reproductive pathogens (Escherichia coli, Pseudomonas aeruginosa, or Klebsiella pneumoniae) isolated from clinical cases. N-acetylcysteine (3.3%) decreased biofilm biomass and killed bacteria within the biofilms of E. coli isolates. The CFU of recoverable P. aeruginosa and K. pneumoniae isolates were decreased, but the biofilm biomass was unchanged. Exposure to hydrogen peroxide (1%) decreased the biofilm biomass and reduced the CFU of E. coli isolates, K. pneumoniae isolates were observed to have a reduction in CFU, and minimal effects were observed for P. aeruginosa isolates. Chelating agents (EDTA formulations) reduced E. coli CFU but were ineffective at disrupting preformed biofilms or decreasing the CFU of P. aeruginosa and K. pneumoniae within a biofilm. No single nonantibiotic treatment commonly used in equine veterinary practice was able to reduce the CFU and biofilm biomass of all three Gram-negative species of bacteria evaluated. An in vivo equine model of infectious endometritis was also developed to monitor biofilm formation, utilizing bioluminescence imaging with equine P. aeruginosa isolates from this study. Following infection, the endometrial surface contained focal areas of bacterial growth encased in a strongly adherent "biofilm-like" matrix, suggesting that biofilms are present during clinical cases of infectious equine endometritis. Our results indicate that Gram-negative bacteria isolated from the equine uterus are capable of producing a biofilm in vitro, and P. aeruginosa is capable of producing biofilm-like material in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.