Evidence map›Paper›PMID 26718871›Full record

ArticleInternational journal of biological macromolecules2016

Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors.

H Zare, A A Moosavi-Movahedi, M Salami, N Sheibani, K Khajeh, M Habibi-Rezaei

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PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of biological macromolecules, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

H ZareInstitute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran; Estahban Fig Research Station, Fars Agricultural and Natural Resources Research and Training Center, AREEO, Shiraz, Iran.
A A Moosavi-MovahediInstitute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran; Center of Excellence in Biothermodynamics (CEBiotherm), University of Tehran, Tehran, Iran. Electronic address: moosavi@ut.ac.ir.
M SalamiInstitute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran; Department of Food Science and Engineering, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran.
N SheibaniDepartment of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA; Department of Pharmacology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
K KhajehFaculty of Biological Sciences, Department of Biochemistry, Tarbiat Modares University, Tehran, Iran.
M Habibi-RezaeiSchool of Biology, University of Tehran, Tehran, Iran.
University of Tehran · IRAgricultural Research & Education Organization · IRTarbiat Modares University · IRUniversity of Wisconsin–Madison · US

Funding

Novel Antiangiogenic Peptides for Treatment of Exudative AMDR24EY022883 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI SHEIBANI, NADER · 2013 to 2017
$6.2M
NEI NIH HHS R24 EY022883
6 · The paper itself

Abstract

The fig's ficin is a cysteine endoproteolytic enzyme, which plays fundamental roles in many plant physiological processes, and has many applications in different industries such as pharmaceutical and food. In this work, we report the inhibition and activation of autolysis and structural changes associated with reaction of ficin with iodoacetamide and tetrathionate using high-performance liquid chromatography (HPLC), ultra filtration membrane, and dynamic light scattering (DLS) methods. The ficin structural changes were also determined using UV-absorption, circular dichroism (CD), fluorescence spectroscopy, and differential scanning calorimetry (DSC) techniques. These techniques demonstrated that iodoacetamide completely inhibited ficin autolysis, which was irreversible. However, tetrathionate partially and reversibility inhibited its autolysis. The ficin structural changes with two synthetic inhibitors were associated with secondary structural changes related to decreased alpha-helix and increased beta sheet and random coil conformations, contributing to its aggregation.

Indexed as

Calorimetry, Differential ScanningChromatography, High Pressure LiquidCircular DichroismEnzyme InhibitorsFicainFicusHydrolysisLatexProtein AggregatesSpectrum AnalysisStructure-Activity RelationshipEnzyme InhibitorsFicainLatexProtein AggregatesAutolysisFicinInhibition

Identifiers

PMID26718871
PMCPMC5223272
OpenAlexW2208916777

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.