Evidence map›Paper›PMID 26718240›Full record

ArticleThe Journal of pharmacology and experimental therapeutics2016

Diisopropylfluorophosphate Impairs the Transport of Membrane-Bound Organelles in Rat Cortical Axons.

Jie Gao, Sean X Naughton, Heike Wulff, Vikrant Singh, Wayne D Beck, Jordi Magrane, Bobby Thomas, Navneet Ammal Kaidery, Caterina M Hernandez, Alvin V Terry

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of pharmacology and experimental therapeutics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
5.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 43 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 1 country.

Jie GaoDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Sean X NaughtonDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Heike WulffDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Vikrant SinghDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Wayne D BeckDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Jordi MagraneDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Bobby ThomasDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Navneet Ammal KaideryDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Caterina M HernandezDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.).
Alvin V TerryDepartment of Pharmacology and Toxicology, Georgia Regents University, Augusta, Georgia (J.G., V.S., S.X.N., W.D.B., B.T., N.A.K., C.M.H., A.V.T.); Department of Pharmacology, University of California-Davis, Davis, California (H.W.); Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, New York (J.M.) aterry@gru.edu.
Weill Cornell Medicine · USGeorgia Regents Medical Center · USAugusta University · USCornell University · USMIND Research Institute · USUniversity of California, Davis · US

Funding

Training and Education CoreU54NS079202 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEIN, PAMELA J · 2012 to 2021
$35.7M
Cholinesterase Inhibitors, Axonal Transport, and MemoryR01ES012241 · NIEHS · UNIVERSITY OF GEORGIA (UGA) · PI TERRY, ALVIN V · 2004 to 2013
$2.8M
NIEHS NIH HHS ES012241NIEHS NIH HHS R01 ES012241NINDS NIH HHS U54 NS079202NINDS NIH HHS U54NS079202
6 · The paper itself

Abstract

The extensive use of organophosphates (OPs) is an ongoing environmental health concern due to multiple reports of OP-related neurologic abnormalities. The mechanism of the acute toxicity of OPs has been attributed to inhibition of acetylcholinesterase (AChE), but there is growing evidence that this may not account for all the long-term neurotoxic effects of OPs. In previous experiments (using ex vivo and in vitro model systems) we observed that the insecticide OP chlorpyrifos impaired the movements of vesicles and mitochondria in axons. Here, using a time-lapse imaging technique, we evaluated the OP-nerve agent diisopropylfluorophosphate (DFP) across a wide range of concentrations (subnanomolar to micromolar) for effects on fast axonal transport of membrane-bound organelles (MBOs) that contain the amyloid precursor protein (APP) tagged with the fluorescent marker Dendra2 (APPDendra2). Both 1 and 24 hours of exposure to DFP and a positive control compound, colchicine, resulted in a decrease in the velocity of anterograde and retrograde movements of MBOs and an increase in the number of stationary MBOs. These effects occurred at picomolar (100 pM) to low nanomolar (0.1 nM) concentrations that were not associated with compromised cell viability or cytoskeletal damage. Moreover, the effects of DFP on axonal transport occurred at concentrations that did not inhibit AChE activity, and they were not blocked by cholinergic receptor antagonists. Given the fundamental importance of axonal transport to neuronal function, these observations may explain some of the long-term neurologic deficits that have been observed in humans who have been exposed to OPs.

Indexed as

AnimalsAxonsBiological TransportCell MembraneCells, CulturedCerebral CortexCholinesterase InhibitorsFemaleIsoflurophateOrganellesPregnancyRatsRats, Sprague-DawleyCholinesterase InhibitorsIsoflurophate

Identifiers

PMID26718240
PMCPMC4767389
OpenAlexW2208034459

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.