ArticleeLife2015
YcgC represents a new protein deacetylase family in prokaryotes.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- FMolecular biology reports · 2023Pooled it
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- c-di-GMP inhibits rRNA methylation and impairs ribosome assembly in the presence of kanamycin.EMBO reports · 2025Article
- Lysine acetylation in cyanobacteria: emerging mechanisms and functions.Biochemical Society transactions · 2025Review
- Protein lysine acetylation regulates oral microorganisms.Frontiers in cellular and infection microbiology · 2025Review
- YkuR functions as a protein deacetylase inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism.Nature communications · 2024Article
- Influence of NMolecules (Basel, Switzerland) · 2024Article
- Acetylomics reveals an extensive acetylation diversity withinmicroLife · 2024Article
- Comparative Lysine Acetylome Analysis of Y. pestis YfiQ/CobB Mutants Reveals that Acetylation of SlyA Lys73 Significantly Promotes Biofilm Formation of Y. pestis.Microbiology spectrum · 2023Article
- Lysine acetylation decreases enzyme activity and protein level ofEngineering microbiology · 2022Article
- Identification of Feronia-interacting proteins in Arabidopsis thaliana.Genes & genomics · 2022Article
- Complete genome sequence of a novel arlivirus from a yellow spotted stink bug (Erthesina fullo (Thunberg, 1783)).Archives of virology · 2022Article
- Systematic analysis of lysine malonylation inFrontiers in cellular and infection microbiology · 2022Article
- Hearing loss and brain plasticity: the hyperactivity phenomenon.Brain structure & function · 2021Review
- Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective.Frontiers in microbiology · 2021Review
- Protein post-translational modifications in bacteria.Nature reviews. Microbiology · 2019Review
- Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP.The EMBO journal · 2019Article
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15 authors at 6 institutions in 3 countries.
Funding
Abstract
Reversible lysine acetylation is one of the most important protein posttranslational modifications that plays essential roles in both prokaryotes and eukaryotes. However, only a few lysine deacetylases (KDACs) have been identified in prokaryotes, perhaps in part due to their limited sequence homology. Herein, we developed a 'clip-chip' strategy to enable unbiased, activity-based discovery of novel KDACs in the Escherichia coli proteome. In-depth biochemical characterization confirmed that YcgC is a serine hydrolase involving Ser200 as the catalytic nucleophile for lysine deacetylation and does not use NAD(+) or Zn(2+) like other established KDACs. Further, in vivo characterization demonstrated that YcgC regulates transcription by catalyzing deacetylation of Lys52 and Lys62 of a transcriptional repressor RutR. Importantly, YcgC targets a distinct set of substrates from the only known E. coli KDAC CobB. Analysis of YcgC's bacterial homologs confirmed that they also exhibit KDAC activity. YcgC thus represents a novel family of prokaryotic KDACs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.