ArticleNature protocols2016
Correlated confocal and super-resolution imaging by VividSTORM.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Article
- Nanoscale synaptic remodeling at corticostriatal circuits predicts flexible action control.bioRxiv : the preprint server for biology · 2025Article
- Dynamic changes of serotonin transporter expression in the prefrontal cortex evoked by aggressive social interactions.Neurobiology of stress · 2025Article
- Presynaptic nanoscale components of retrograde synaptic signaling.Science advances · 2024Article
- SuperCUT, an unsupervised multimodal image registration with deep learning for biomedical microscopy.Briefings in bioinformatics · 2024Article
- Brain-Targeted Liposomes Loaded with Monoclonal Antibodies Reduce Alpha-Synuclein Aggregation and Improve Behavioral Symptoms in Parkinson's Disease.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Unique features of the arterial blood-brain barrier.Fluids and barriers of the CNS · 2023Article
- STORM Super-Resolution Imaging of CBMethods in molecular biology (Clifton, N.J.) · 2023Article
- Discrimination of normal and cancerous human skin tissues based on laser-induced spectral shift fluorescence microscopy.Scientific reports · 2022Article
- Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies.International journal of molecular sciences · 2022Article
- A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.Nature communications · 2022Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Imaging the endocannabinoid signaling system.Journal of neuroscience methods · 2022Review
- Article
- PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells.Nature communications · 2021Article
- The cardiac nanoenvironment: form and function at the nanoscale.Biophysical reviews · 2021Review
- Parametric comparison between sparsity-based and deep learning-based image reconstruction of super-resolution fluorescence microscopy.Biomedical optics express · 2021Article
- Robust adaptive optics for localization microscopy deep in complex tissue.Nature communications · 2021Article
- NECAB1 and NECAB2 are Prevalent Calcium-Binding Proteins of CB1/CCK-Positive GABAergic Interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Single-molecule localization microscopy (SMLM) is rapidly gaining popularity in the life sciences as an efficient approach to visualize molecular distribution with nanoscale precision. However, it has been challenging to obtain and analyze such data within a cellular context in tissue preparations. Here we describe a 5-d tissue processing and immunostaining procedure that is optimized for SMLM, and we provide example applications to fixed mouse brain, heart and kidney tissues. We then describe how to perform correlated confocal and 3D-superresolution imaging on these sections, which allows the visualization of nanoscale protein localization within labeled subcellular compartments of identified target cells in a few minutes. Finally, we describe the use of VividSTORM (http://katonalab.hu/index.php/vividstorm), an open-source software for correlated confocal and SMLM image analysis, which facilitates the measurement of molecular abundance, clustering, internalization, surface density and intermolecular distances in a cell-specific and subcellular compartment-restricted manner. The protocol requires only basic skills in tissue staining and microscopy.
Indexed as
Identifiers
26716705What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.