Evidence map›Paper›PMID 26715821›Full record

Observational studyWorld journal of gastroenterology2015

Development of Fok-I based nested polymerase chain reaction-restriction fragment length polymorphism analysis for detection of hepatitis B virus X region V5M mutation.

Hong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim

Open access · hybridAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in World journal of gastroenterology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Hong KimHong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea.
Seok-Hyun HongHong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea.
Seoung-Ae LeeHong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea.
Jeong-Ryeol GongHong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea.
Bum-Joon KimHong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea.
Seoul National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo develop a Fok-I nested polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) method for the detection of hepatitis B virus X region (HBx) V5M mutation.

methodsNested PCR was applied into DNAs from 198 chronic patients at 2 different stages [121 patients with hepatocellular carcinoma (HCC) and 77 carrier patients]. To identify V5M mutants, digestion of nested PCR amplicons by the restriction enzyme Fok-I (GGA TGN9↓) was done. For size comparison, the enzyme-treated products were analyzed by electrophoresis on 2.5% agarose gels, stained with ethidium bromide, and visualized on a UV transilluminator.

resultsThe assay enabled the identification of 69 patients (sensitivity of 34.8%; 46 HCC patients and 23 carrier patients). Our data also showed that V5M prevalence in HCC patients was significantly higher than in carrier patients (47.8%, 22/46 patients vs 0%, 0/23 patients, P < 0.001), suggesting that HBxAg V5M mutation may play a pivotal role in HCC generation in chronic patients with genotype C infections.

conclusionThe Fok-I nested PRA developed in this study is a reliable and cost-effective method to detect HBxAg V5M mutation in chronic patients with genotype C2 infection.

Indexed as

MutationPolymorphism, Restriction Fragment LengthAmplified Fragment Length Polymorphism AnalysisAsian PeopleCarcinoma, HepatocellularDeoxyribonucleases, Type II Site-SpecificDisease ProgressionDNA Mutational AnalysisGene FrequencyGenetic Predisposition to DiseaseHepatitis B, ChronicHepatitis B virusHumansLiver NeoplasmsPhenotypePolymerase Chain ReactionDeoxyribonucleases, Type II Site-Specificendodeoxyribonuclease FokIhepatitis B virus X proteinTrans-ActivatorsViral Regulatory and Accessory ProteinsHepatitis B virusHepatocellur carcinomaPolymerase chain reaction-restriction fragment length polymorphism analysisV5M mutationX antigen

Identifiers

PMID26715821
PMCPMC4679770
OpenAlexW2413176033

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.