ArticleProceedings of the National Academy of Sciences of the United States of America2016
In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 123 citations in OpenAlex.
- Structurally diverse acylsugars on wild tomatillo fruit surfaces.Horticulture research · 2026Article
- Evolutionary hijacking of fatty acid biosynthesis drives insecticidal acylsugar diversity in tomato.Nature communications · 2026Article
- The organization of acylsugar biosynthetic enzymes: what is next for metabolic efficiency in plant defense?Journal of experimental botany · 2026Article
- A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis.Journal of experimental botany · 2026Article
- The plasma membrane ABC transporter Sl-ABCB5 mediates acylsugar secretion from tomato trichomes to confer insect resistance.Plant communications · 2026Article
- Conservation of IAMT Preference for Indole Acetic Acid Methylation Across 250 Million Years of Seed Plant Divergence, With Only One Recent Evolutionary Switch in Ocimum.Genome biology and evolution · 2026Article
- Engineering carotenoid and steroidal glycoalkaloid depleted tomato fruit for heterologous production of high value terpenes.bioRxiv : the preprint server for biology · 2026Article
- Functional diversification of WRINKLED3 integrates fatty acid metabolism with insecticidal acylsugar production in Solanaceae species.Nature communications · 2026Article
- Conserved differential expression of DDX helicase and CPEB genes in skeletal muscles potentially influences neuromuscular junctions during aging: a computational analysis.Biogerontology · 2026Article
- Tomato and its relatives are breaking the genomics barriers.Journal of experimental botany · 2025Review
- Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.The New phytologist · 2025Article
- Phylogenomic and synteny analysis of BAHD and SCP/SCPL gene families reveal their evolutionary histories in plant specialized metabolism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Two ubiquitous aldo-keto reductases in the genus Papaver support a patchwork model for morphine pathway evolution.Communications biology · 2024Article
- Trading acyls and swapping sugars: metabolic innovations in Solanum trichomes.Plant physiology · 2024Article
- A tale of sugars' tails-diverse acyl chains decorate sugars in Solanum species' trichomes.Plant physiology · 2024Article
- Tomato root specialized metabolites evolved through gene duplication and regulatory divergence within a biosynthetic gene cluster.Science advances · 2024Article
- Trading acyls and swapping sugars: metabolic innovations inbioRxiv : the preprint server for biology · 2024Article
- A BAHD-type acyltransferase concludes the biosynthetic pathway of non-bitter glycoalkaloids in ripe tomato fruit.Nature communications · 2023Article
- Specialized metabolism by trichome-enriched Rubisco and fatty acid synthase components.Plant physiology · 2023Article
- Genomic and metabolic profiling of two tomato contrasting cultivars for tolerance to Tuta absoluta.Planta · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant glandular secreting trichomes are epidermal protuberances that produce structurally diverse specialized metabolites, including medically important compounds. Trichomes of many plants in the nightshade family (Solanaceae) produce O-acylsugars, and in cultivated and wild tomatoes these are mixtures of aliphatic esters of sucrose and glucose of varying structures and quantities documented to contribute to insect defense. We characterized the first two enzymes of acylsucrose biosynthesis in the cultivated tomato Solanum lycopersicum. These are type I/IV trichome-expressed BAHD acyltransferases encoded by Solyc12g006330--or S. lycopersicum acylsucrose acyltransferase 1 (Sl-ASAT1)--and Solyc04g012020 (Sl-ASAT2). These enzymes were used--in concert with two previously identified BAHD acyltransferases--to reconstruct the entire cultivated tomato acylsucrose biosynthetic pathway in vitro using sucrose and acyl-CoA substrates. Comparative genomics and biochemical analysis of ASAT enzymes were combined with in vitro mutagenesis to identify amino acids that influence CoA ester substrate specificity and contribute to differences in types of acylsucroses that accumulate in cultivated and wild tomato species. This work demonstrates the feasibility of the metabolic engineering of these insecticidal metabolites in plants and microbes.
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