ArticleMicrobiome2015
A simple novel device for air sampling by electrokinetic capture.
Article in Microbiome, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- The indoors microbiome and human health.Nature reviews. Microbiology · 2024Review
- Assessment of a Program for SARS-CoV-2 Screening and Environmental Monitoring in an Urban Public School District.JAMA network open · 2021Article
- Miniaturizing Wet Scrubbers for Aerosolized Droplet Capture.Analytical chemistry · 2021Article
- Bioaerosol Sampling: Classical Approaches, Advances, and Perspectives.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2020Article
- Concurrent measurement of microbiome and allergens in the air of bedrooms of allergy disease patients in the Chicago area.Microbiome · 2019Article
- Crawling-induced floor dust resuspension affects the microbiota of the infant breathing zone.Microbiome · 2018Article
- Field Performance of a Novel Passive Bioaerosol Sampler using Polarized Ferroelectric Polymer Films.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable.
resultsAn air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87 %, with the reference filter taken as "gold standard." Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air.
conclusionsThis work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.