ArticleParasites & vectors2015
Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi.Communications biology · 2025Article
- Mitochondrial DNA Structure inPathogens (Basel, Switzerland) · 2025Review
- Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage.PloS one · 2025Article
- Article
- A phased genome assembly of a Colombian Trypanosoma cruzi TcI strain and the evolution of gene families.Scientific reports · 2024Article
- Hydrophobicity-Driven Increases in Editing in Mitochondrial mRNAs during the Evolution of Kinetoplastids.Molecular biology and evolution · 2023Article
- Novel insertions in the mitochondrial maxicircle ofParasitology · 2022Article
- Article
- Axenic interspecies and intraclonal hybrid formation in Leishmania: Successful crossings between visceral and cutaneous strains.PLoS neglected tropical diseases · 2022Article
- Mitochondrial RNA editing inComputational and structural biotechnology journal · 2022Article
- rKOMICS: an R package for processing mitochondrial minicircle assemblies in population-scale genome projects.BMC bioinformatics · 2021Article
- Reproduction in Trypanosomatids: Past and Present.Biology · 2021Review
- Infection withParasitology · 2021Article
- Guide RNA Repertoires in the Main Lineages ofFrontiers in cellular and infection microbiology · 2021Article
- Novel organization of mitochondrial minicircles and guide RNAs in the zoonotic pathogen Trypanosoma lewisi.Nucleic acids research · 2020Article
- Common Structural Patterns in the Maxicircle Divergent Region of Trypanosomatidae.Pathogens (Basel, Switzerland) · 2020Article
- Identification of trypanosomatids and blood feeding preferences of phlebotomine sand fly species common in Sicily, Southern Italy.PloS one · 2020Article
- Biological attributes of the kissing bug Triatoma rubrofasciata from Vietnam.Parasites & vectors · 2019Article
- Article
- Evolutionary Insight into the Trypanosomatidae Using Alignment-Free Phylogenomics of the Kinetoplast.Pathogens (Basel, Switzerland) · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe haemoflagellate Trypanosoma lewisi is a kinetoplastid parasite which, as it has been recently reported to cause human disease, deserves increased attention. Characteristic features of all kinetoplastid flagellates are a uniquely structured mitochondrial DNA or kinetoplast, comprised of a network of catenated DNA circles, and RNA editing of mitochondrial transcripts. The aim of this study was to describe the kinetoplast DNA of T. lewisi. METHODS/
resultsIn this study, purified kinetoplast DNA from T. lewisi was sequenced using high-throughput sequencing in combination with sequencing of PCR amplicons. This allowed the assembly of the T. lewisi kinetoplast maxicircle DNA, which is a homologue of the mitochondrial genome in other eukaryotes. The assembly of 23,745 bp comprises the non-coding and coding regions. Comparative analysis of the maxicircle sequence of T. lewisi with Trypanosoma cruzi, Trypanosoma rangeli, Trypanosoma brucei and Leishmania tarentolae revealed that it shares 78%, 77%, 74% and 66% sequence identity with these parasites, respectively. The high GC content in at least 9 maxicircle genes of T. lewisi (ATPase6; NADH dehydrogenase subunits ND3, ND7, ND8 and ND9; G-rich regions GR3 and GR4; cytochrome oxidase subunit COIII and ribosomal protein RPS12) implies that their products may be extensively edited. A detailed analysis of the non-coding region revealed that it contains numerous repeat motifs and palindromes.
conclusionsWe have sequenced and comprehensively annotated the kinetoplast maxicircle of T. lewisi. Our analysis reveals that T. lewisi is closely related to T. cruzi and T. brucei, and may share similar RNA editing patterns with them rather than with L. tarentolae. These findings provide novel insight into the biological features of this emerging human pathogen.
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