ArticleSleep2016
Loss of Sleep Affects the Ultrastructure of Pyramidal Neurons in the Adolescent Mouse Frontal Cortex.
Article in Sleep, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 49 citations in OpenAlex.
- Repeated caffeine intake suppresses cerebral grey matter responses to chronic sleep restriction in an AScientific reports · 2024Trial
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- Sleep as a moderator of adolescent brain development-evidence from a longitudinal MRI study.Sleep · 2026Article
- Spatiotemporally dynamic noradrenergic regulation of cortical networks.bioRxiv : the preprint server for biology · 2026Article
- A hippocampal 'sharp-wave sleep' state that is dissociable from cortical sleep.Nature neuroscience · 2026Article
- Simvastatin modulates sleep-wake behavior and locomotor activity in a dose-dependent manner inFrontiers in pharmacology · 2026Article
- Neurobiology of mitochondrial dynamics in sleep.The Journal of physiology · 2025Review
- Mitochondrial origins of the pressure to sleep.Nature · 2025Article
- Brief sleep disruption following hippocampus-dependent learning downscales interneuron synapses within lateral entorhinal cortex.bioRxiv : the preprint server for biology · 2025Article
- Molecular Effects of Cornelian Cherry Fruit (Current issues in molecular biology · 2025Article
- Introduction of novel objects to the home cage of mice repeatedly disturbs sleep for seven days with minimal stress induction.Npj biological timing and sleep · 2025Article
- Phylogenetic conservation of the interdependent homeostatic relationship of sleep regulation and redox metabolism.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- CorticalProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Loss of sleep when it is needed most - Consequences of persistent developmental sleep disruption: A scoping review of rodent models.Neurobiology of sleep and circadian rhythms · 2023Article
- Sleep deficiency as a driver of cellular stress and damage in neurological disorders.Sleep medicine reviews · 2022Review
- Perspective - ultrastructural analyses reflect the effects of sleep and sleep loss on neuronal cell biology.Sleep · 2022Article
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- The Ontogenesis of Mammalian Sleep: Form and Function.Current sleep medicine reports · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
STUDY
objectiveThe adolescent brain may be uniquely affected by acute sleep deprivation (ASD) and chronic sleep restriction (CSR), but direct evidence is lacking. We used electron microscopy to examine how ASD and CSR affect pyramidal neurons in the frontal cortex of adolescent mice, focusing on mitochondria, endosomes, and lysosomes that together perform most basic cellular functions, from nutrient intake to prevention of cellular stress.
methodsAdolescent (1-mo-old) mice slept (S) or were sleep deprived (ASD, with novel objects and running wheels) during the first 6-8 h of the light period, chronically sleep restricted (CSR) for > 4 days (using novel objects, running wheels, social interaction, forced locomotion, caffeinated water), or allowed to recover sleep (RS) for ∼32 h after CSR. Ultrastructural analysis of 350 pyramidal neurons was performed (S = 82; ASD = 86; CSR = 103; RS = 79; 4 to 5 mice/group).
resultsSeveral ultrastructural parameters differed in S versus ASD, S versus CSR, CSR versus RS, and S versus RS, although the different methods used to enforce wake may have contributed to some of the differences between short and long sleep loss. Differences included larger cytoplasmic area occupied by mitochondria in CSR versus S, and higher number of secondary lysosomes in CSR versus S and RS. We also found that sleep loss may unmask interindividual differences not obvious during baseline sleep. Moreover, using a combination of 11 ultrastructural parameters, we could predict in up to 80% of cases whether sleep or wake occurred at the single cell level.
conclusionsUltrastructural analysis may be a powerful tool to identify which cellular organelles, and thus which cellular functions, are most affected by sleep and sleep loss.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.