ArticleMolecular microbiology2016
Structural and functional analysis of RopB: a major virulence regulator in Streptococcus pyogenes.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- The leaderless communication peptide (LCP) class of quorum-sensing peptides is broadly distributed among Firmicutes.Nature communications · 2023Article
- RopB represses the transcription ofLife science alliance · 2023Article
- Streptococcal peptides and their roles in host-microbe interactions.Frontiers in cellular and infection microbiology · 2023Review
- Streptococcus pyogenes genes that promote pharyngitis in primates.JCI insight · 2020Article
- In silico characterisation of stand-alone response regulators of Streptococcus pyogenes.PloS one · 2020Article
- Environmental pH and peptide signaling control virulence of Streptococcus pyogenes via a quorum-sensing pathway.Nature communications · 2019Article
- Deciphering the Molecular Mechanism Underpinning Phage Arbitrium Communication Systems.Molecular cell · 2019Article
- Article
- Structural basis of the arbitrium peptide-AimR communication system in the phage lysis-lysogeny decision.Nature microbiology · 2018Article
- Signaling by a Conserved Quorum Sensing Pathway Contributes to GrowthInfection and immunity · 2018Article
- Endopeptidase PepO Regulates the SpeB Cysteine Protease and Is Essential for the Virulence of Invasive M1T1 Streptococcus pyogenes.Journal of bacteriology · 2018Article
- A novel chemical inducer ofThe Journal of biological chemistry · 2018Article
- Genetic and Structural Analyses of RRNPP Intercellular Peptide Signaling of Gram-Positive Bacteria.Annual review of genetics · 2017Review
- Leaderless secreted peptide signaling molecule alters global gene expression and increases virulence of a human bacterial pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS.PLoS pathogens · 2016Article
- Pheromone Recognition and Selectivity by ComR Proteins among Streptococcus Species.PLoS pathogens · 2016Article
- Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators.Journal of molecular biology · 2016Review
- Intergenic Variable-Number Tandem-Repeat Polymorphism Upstream of rocA Alters Toxin Production and Enhances Virulence in Streptococcus pyogenes.Infection and immunity · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Group A Streptococcus (GAS) is an exclusive human pathogen that causes significant disease burden. Global regulator RopB of GAS controls the expression of several major virulence factors including secreted protease SpeB during high cell density. However, the molecular mechanism for RopB-dependent speB expression remains unclear. To understand the mechanism of transcription activation by RopB, we determined the crystal structure of the C-terminal domain of RopB. RopB-CTD has the TPR motif, a signature motif involved in protein-peptide interactions and shares significant structural homology with the quorum sensing RRNPP family regulators. Characterization of the high cell density-specific cell-free growth medium demonstrated the presence of a low molecular weight proteinaceous secreted factor that upregulates RopB-dependent speB expression. Together, these results suggest that RopB and its cognate peptide signals constitute an intercellular signalling machinery that controls the virulence gene expression in concert with population density. Structure-guided mutational analyses of RopB dimer interface demonstrated that single alanine substitutions at this critical interface significantly altered RopB-dependent speB expression and attenuated GAS virulence. Results presented here suggested that a properly aligned RopB dimer interface is important for GAS pathogenesis and highlighted the dimerization interactions as a plausible therapeutic target for the development of novel antimicrobials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.