ArticlePloS one2015
The Effect of Lactobacillus casei 32G on the Mouse Cecum Microbiota and Innate Immune Response Is Dose and Time Dependent.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Polyphenol-modified probiotics for the enhanced survivability, persistence and colonization of probiotic cells in the gut.Current research in food science · 2026Article
- Lacticaseibacillus casei CB054 short-term supplementation effect on murine splenocyte cytokine transcription and the gut bacterial microbiota.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- A neurotherapeutic approach with Lacticaseibacillus rhamnosus E9 on gut microbiota and intestinal barrier in MPTP-induced mouse model of Parkinson's disease.Scientific reports · 2024Article
- Identification of novel probiotic lactic acid bacteria from soymilk waste using the 16s rRNA gene for potential use in poultry.Veterinary world · 2024Article
- The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties.Microorganisms · 2024Review
- Gut Microbial Alteration in MPTP Mouse Model of Parkinson Disease is Administration Regimen Dependent.Cellular and molecular neurobiology · 2023Article
- Alterations in Faecal Microbiota and Elevated Levels of Intestinal IgA Following Oral Administration of Lacticaseibacillus casei in mice.Probiotics and antimicrobial proteins · 2023Article
- Probiotics enhances anti-tumor immune response induced by gemcitabine plus cisplatin chemotherapy for urothelial cancer.Cancer science · 2023Article
- Influence of fermented feed additive on gut morphology, immune status, and microbiota in broilers.BMC veterinary research · 2022Article
- Lactobacillus rhamnosus CGMCC 1.3724 (LPR) Improves Skin Wound Healing and Reduces Scar Formation in Mice.Probiotics and antimicrobial proteins · 2021Article
- The Role of Vasoactive Intestinal Peptide and Mast Cells in the Regulatory Effect ofFrontiers in immunology · 2021Article
- Proteomic analysis ofIranian journal of microbiology · 2020Article
- Probiotic from human breast milk, Lactobacillus fermentum, promotes growth in animal model of chronic malnutrition.Pediatric research · 2020Article
- Recent Advancements in the Development of Modern Probiotics for Restoring Human Gut Microbiome Dysbiosis.Indian journal of microbiology · 2020Review
- Lactobacilli Isolated From Wild Boar (Frontiers in microbiology · 2019Article
- Effects ofAsian-Australasian journal of animal sciences · 2018Article
- Emerging Developments in Microbiome and Microglia Research: Implications for Neurodevelopmental Disorders.Frontiers in immunology · 2018Review
- Oral Administration of Probiotics Increases Paneth Cells and Intestinal Antimicrobial Activity.Frontiers in microbiology · 2018Article
- In silico analysis of protein toxin and bacteriocins from Lactobacillus paracasei SD1 genome and available online databases.PloS one · 2017Article
- Harnessing bacteriocin biology as targeted therapy in the GI tract.Gut microbes · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacilli have been associated with a variety of immunomodulatory effects and some of these effects have been related to changes in gastrointestinal microbiota. However, the relationship between probiotic dose, time since probiotic consumption, changes in the microbiota, and immune system requires further investigation. The objective of this study was to determine if the effect of Lactobacillus casei 32G on the murine gastrointestinal microbiota and immune function are dose and time dependent. Mice were fed L. casei 32G at doses of 106, 107, or 108 CFU/day/mouse for seven days and were sacrificed 0.5h, 3.5h, 12h, or 24h after the last administration. The ileum tissue and the cecal content were collected for immune profiling by qPCR and microbiota analysis, respectively. The time required for L. casei 32G to reach the cecum was monitored by qPCR and the 32G bolus reaches the cecum 3.5h after the last administration. L. casei 32G altered the cecal microbiota with the predominance of Lachnospiraceae IS, and Oscillospira decreasing significantly (p < 0.05) in the mice receiving 108 CFU/mouse 32G relative to the control mice, while a significant (p < 0.05) increase was observed in the prevalence of lactobacilli. The lactobacilli that increased were determined to be a commensal lactobacilli. Interestingly, no significant difference in the overall microbiota composition, regardless of 32G doses, was observed at the 12h time point. A likely explanation for this observation is the level of feed derived-nutrients resulting from the 12h light/dark cycle. 32G results in consistent increases in Clec2h expression and reductions in TLR-2, alpha-defensins, and lysozyme. Changes in expression of these components of the innate immune system are one possible explanation for the observed changes in the cecal microbiota. Additionally, 32G administration was observed to alter the expression of cytokines (IL-10rb and TNF-α) in a manner consistent with an anti-inflammatory response.
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