ArticleJournal of experimental botany2016
Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 39 citations in OpenAlex.
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- Genomic prediction reveals unexplored variation in grain protein and lysine content across a vast winter wheat genebank collection.Frontiers in plant science · 2023Article
- Multi-omics approach highlights new targets for amino acid composition change in maize kernels.Plant physiology · 2022Article
- Multiomics approach reveals a role of translational machinery in shaping maize kernel amino acid composition.Plant physiology · 2022Article
- StresSeed: The Unfolded Protein Response During Seed Development.Frontiers in plant science · 2022Review
- The Unique Seed Protein Composition of Quality Protein Popcorn Promotes Growth of Beneficial Bacteria From the Human Gut Microbiome.Frontiers in microbiology · 2022Article
- Temporal profiling of essential amino acids in developing maize kernel of normal,Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Intra-Kernel Reallocation of Proteins in Maize Depends on VP1-Mediated Scutellum Development and Nutrient Assimilation.The Plant cell · 2019Article
- Article
- Editing of an Alpha-Kafirin Gene Family Increases, Digestibility and Protein Quality in Sorghum.Plant physiology · 2018Article
- Opaque16, a high lysine and tryptophan mutant, does not influence the key physico-biochemical characteristics in maize kernel.PloS one · 2018Article
- Generation and Evaluation of ModifiedFrontiers in plant science · 2018Article
- Shotgun proteomics of the barley seed proteome.BMC genomics · 2017Article
- Responses to Hypoxia and Endoplasmic Reticulum Stress Discriminate the Development of Vitreous and Floury Endosperms of Conventional Maize (Frontiers in plant science · 2017Article
- Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.Journal of experimental botany · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, and proteolysis. Principal component analysis and pathway enrichment suggested that the mutants partitioned into three groups: (i) Mc, DeB30, fl2 and o2; (ii) o1; and (iii) fl1. Indicator species analysis revealed mutant-specific proteins, and highlighted ER secretory pathway components that were enriched in selected groups of mutants. The most significantly changed proteins were related to stress or defense and zein partitioning into the soluble fraction for Mc, DeB30, o1, and fl1 specifically. In silico dissection of the most significantly changed proteins revealed novel qualitative changes in lysine abundance contributing to the overall lysine increase and the nutritional rebalancing of the o2 and fl2 endosperm.
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