ArticleCell stem cell2016
Human-Mouse Chimerism Validates Human Stem Cell Pluripotency.
Article in Cell stem cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 118 citations in OpenAlex.
- Article
- A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures.Nature cell biology · 2026Article
- Xenophagocytosis blockade enhances interspecies chimerism.bioRxiv : the preprint server for biology · 2025Article
- CRISPR-edited iPSCs reveal BSN gene mutations induce neuronal hyperexcitability via astrocyte lipid accumulation.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Article
- Blastocyst complementation: current progress and future directions in xenogeneic organogenesis.Stem cell research & therapy · 2025Review
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- Article
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing.Stem cell reports · 2023Article
- Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools.Nature communications · 2023Article
- Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy.Frontiers in cell and developmental biology · 2023Review
- Review
- Review
- Genome-wide screening identifies Polycomb repressive complex 1.3 as an essential regulator of human naïve pluripotent cell reprogramming.Science advances · 2022Article
- Glutamine-dependent signaling controls pluripotent stem cell fate.Developmental cell · 2022Article
- Fitness selection in human pluripotent stem cells and interspecies chimeras: Implications for human development and regenerative medicine.Developmental biology · 2021Review
- The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras.Development (Cambridge, England) · 2021Article
- Modeling human embryo development with embryonic and extra-embryonic stem cells.Developmental biology · 2021Review
- Running the full human developmental clock in interspecies chimeras using alternative human stem cells with expanded embryonic potential.NPJ Regenerative medicine · 2021Review
- Interspecies chimeric conditions affect the developmental rate of human pluripotent stem cells.PLoS computational biology · 2021Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Pluripotent stem cells are defined by their capacity to differentiate into all three tissue layers that comprise the body. Chimera formation, generated by stem cell transplantation to the embryo, is a stringent assessment of stem cell pluripotency. However, the ability of human pluripotent stem cells (hPSCs) to form embryonic chimeras remains in question. Here we show using a stage-matching approach that human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) have the capacity to participate in normal mouse development when transplanted into gastrula-stage embryos, providing in vivo functional validation of hPSC pluripotency. hiPSCs and hESCs form interspecies chimeras with high efficiency, colonize the embryo in a manner predicted from classical developmental fate mapping, and differentiate into each of the three primary tissue layers. This faithful recapitulation of tissue-specific fate post-transplantation underscores the functional potential of hPSCs and provides evidence that human-mouse interspecies developmental competency can occur.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.