ArticleApplied and environmental microbiology2015
Visualizing Bdellovibrio bacteriovorus by Using the tdTomato Fluorescent Protein.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- From microbes to animals: a review on prey choice and prey-predator dynamics across organismal scales.FEMS microbiology reviews · 2026Review
- A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus.PLoS genetics · 2025Article
- Article
- An efficient method for visualizing the plaques of Bdellovibrio bacteriovorus.Archives of microbiology · 2024Article
- Predation on bacterial pathogens by predatory bacteria of sewage origin: three days prey-predator interactions.BMC microbiology · 2024Article
- Article
- Controlling the expression of heterologous genes in Bdellovibrio bacteriovorus using synthetic biology strategies.Microbial biotechnology · 2024Article
- Self-demise of soft rot bacteria by activation of microbial predators by pectin-based carriers.Microbial biotechnology · 2023Article
- An MltA-Like Lytic Transglycosylase Secreted by Bdellovibrio bacteriovorus Cleaves the Prey Septum during Predatory Invasion.Journal of bacteriology · 2023Article
- Article
- Use of Resazurin To Rapidly EnumerateMicrobiology spectrum · 2022Article
- Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria.Nature communications · 2022Article
- An optimized workflow to measure bacterial predation in microplates.STAR protocols · 2022Article
- Validating Flow Cytometry as a Method for QuantifyingMicrobiology spectrum · 2022Article
- Bacterial predation transforms the landscape and community assembly of biofilms.Current biology : CB · 2021Article
- Quinolone Signals Related toFrontiers in microbiology · 2021Article
- To hunt or to rest: prey depletion induces a novel starvation survival strategy in bacterial predators.The ISME journal · 2021Article
- Xylose-Inducible Promoter Tools forApplied and environmental microbiology · 2020Article
- Potential Control of Potato Soft Rot Disease by the Obligate PredatorsApplied and environmental microbiology · 2020Article
- Biotechnological Potential ofFrontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Bdellovibrio bacteriovorus is a Gram-negative bacterium that belongs to the delta subgroup of proteobacteria and is characterized by a predatory life cycle. In recent years, work has highlighted the potential use of this predator to control bacteria and biofilms. Traditionally, the reduction in prey cells was used to monitor predation dynamics. In this study, we introduced pMQ414, a plasmid that expresses the tdTomato fluorescent reporter protein, into a host-independent strain and a host-dependent strain of B. bacteriovorus 109J. The new construct was used to conveniently monitor predator proliferation in real time, in different growth conditions, in the presence of lytic enzymes, and on several prey bacteria, replicating previous studies that used plaque analysis to quantify B. bacteriovorus. The new fluorescent plasmid also enabled us to visualize the predator in liquid cultures, in the context of a biofilm, and in association with human epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.