ArticleApplied and environmental microbiology2015
Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Protection of macrophages from intracellular pathogens by miR-182-5p mimic-a gene expression meta-analysis approach.The FEBS journal · 2018Pooled it
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- Review
- Studies of Streptococcus anginosus Virulence in Dictyostelium discoideum and Galleria mellonella Models.Infection and immunity · 2023Article
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- Amoebae can promote the survival ofEmerging microbes & infections · 2021Article
- Disruption of the Francisella noatunensis subsp.Infection and immunity · 2021Article
- Type VI Secretion System and Its Effectors PdpC, PdpD, and OpiA Contribute toInfection and immunity · 2021Article
- Marine Bacteria Display Different Escape Mechanisms When Facing Their Protozoan Predators.Microorganisms · 2020Article
- Larva of greater wax moth Galleria mellonella is a suitable alternative host for the fish pathogen Francisella noatunensis subsp. orientalis.BMC microbiology · 2020Article
- Optimisation of External Factors for the Growth ofBioMed research international · 2020Article
- Tularemia as a waterborne disease: a review.Emerging microbes & infections · 2019Review
- Quantitative Imaging Flow Cytometry of Legionella-Infected Dictyostelium Amoebae Reveals the Impact of Retrograde Trafficking on Pathogen Vacuole Composition.Applied and environmental microbiology · 2018Article
- Inorganic Polyphosphate Is Essential forFrontiers in cellular and infection microbiology · 2018Article
- Characterization and Vaccine Potential of Membrane Vesicles Produced by Francisella noatunensis subsp. orientalis in an Adult Zebrafish Model.Clinical and vaccine immunology : CVI · 2017Article
- Autophagy in Dictyostelium: Mechanisms, regulation and disease in a simple biomedical model.Autophagy · 2017Review
- When Dicty Met Myco, a (Not So) Romantic Story about One Amoeba and Its Intracellular Pathogen.Frontiers in cellular and infection microbiology · 2017Review
- Eat Prey, Live:Frontiers in immunology · 2017Review
- Nramp1 and NrampB Contribute to Resistance againstFrontiers in cellular and infection microbiology · 2017Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Francisella bacteria cause severe disease in both vertebrates and invertebrates and include one of the most infectious human pathogens. Mammalian cell lines have mainly been used to study the mechanisms by which Francisella manipulates its host to replicate within a large variety of hosts and cell types, including macrophages. Here, we describe the establishment of a genetically and biochemically tractable infection model: the amoeba Dictyostelium discoideum combined with the fish pathogen Francisella noatunensis subsp. noatunensis. Phagocytosed F. noatunensis subsp. noatunensis interacts with the endosomal pathway and escapes further phagosomal maturation by translocating into the host cell cytosol. F. noatunensis subsp. noatunensis lacking IglC, a known virulence determinant required for Francisella intracellular replication, follows the normal phagosomal maturation and does not grow in Dictyostelium. The attenuation of the F. noatunensis subsp. noatunensis ΔiglC mutant was confirmed in a zebrafish embryo model, where growth of F. noatunensis subsp. noatunensis ΔiglC was restricted. In Dictyostelium, F. noatunensis subsp. noatunensis interacts with the autophagic machinery. The intracellular bacteria colocalize with autophagic markers, and when autophagy is impaired (Dictyostelium Δatg1), F. noatunensis subsp. noatunensis accumulates within Dictyostelium cells. Altogether, the Dictyostelium-F. noatunensis subsp. noatunensis infection model recapitulates the course of infection described in other host systems. The genetic and biochemical tractability of the system allows new approaches to elucidate the dynamic interactions between pathogenic Francisella and its host organism.
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